Thioesterases and cells for production of tailored oils
The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.
1. A recombinant nucleic acid construct comprising a regulatory element and a FatB gene expressing an active acyl-ACP thioesterase operable to produce an altered fatty acid profile in an oil produced by a cell expressing the nucleic acid construct, wherein the FatB gene expresses a protein having at least 94.6%, 95%, 96%, 97%, 98%, or 99% sequence identity with SEQ ID NOs: 82 or 178, and optionally wherein the fatty acid of the oil is enriched in C8 and C10 fatty acids.
2. An isolated nucleic acid or recombinant DNA construct comprising a nucleic acid, wherein the nucleic acid has at least 95%, 96%, 97%, 98%, or 99% sequence identity to any of SEQ ID NOS: 83, 84, 90, or any equivalent sequences by virtue of the degeneracy of the genetic code, wherein the isolated nucleic acid or recombinant DNA construct encodes an active acyl-ACP thiosterase operable to produce an altered fatty acid profile in an oil produced by a cell expressing said isolated nucleic acid or recombinant DNA construct.
3. An isolated nucleic acid sequence encoding a protein or a host cell expressing a protein having at least 95%, 96%, 97%, 98%, or 99% sequence identity to any of SEQ ID NOS: 82, 178 or a fragment thereof, said isolated nucleic acid sequence encoding a protein having acyl-ACP thioesterase activity.
4. The isolated nucleic acid of claim 3 , wherein, the protein has acyl-ACP thioesterase activity operable to alter the fatty acid profile of an oil produced by a recombinant cell comprising that sequence.
5. A method of producing a recombinant cell that produces an altered fatty acid profile, the method comprising transforming the cell with a nucleic acid construct according to claim 1 .
6. A host cell produced by the method of claim 5 .
7. The host cell of claim 6 , wherein the host cell is selected from a plant cell, a microbial cell, and a microalgal cell.
8. A method for producing an oil or oil-derived product, the method comprising cultivating a host cell of claim 6 , and extracting oil produced thereby, optionally wherein the cultivation is heterotrophic growth on sugar.
9. The method of claim 8 , further comprising producing a fatty acid, fuel, chemical, or other oil-derived product from the oil.