COMPOSITIONS AND METHODS FOR EXPRESSING TRANSGENES USING REGULATORY ELEMENTS FROM RUBISCO ACTIVASE GENES
Provided are compositions and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters, 5′UTR, 3′ UTRs, and/or terminators isolated from Glycine max rubisco activase genes.
1 . A nucleic acid expression cassette, comprising a promoter operably linked to a non-rubisco activase transgene, wherein said promoter comprises SEQ ID NO:1 or SEQ ID NO:6, or a sequence that has 95% sequence identity with SEQ ID NO:1 or SEQ ID NO:6.
2 . (canceled)
3 . The nucleic acid expression cassette of claim 1 , further comprising a 5′ untranslated region comprising SEQ ID NO:2 or SEQ ID NO:7, or a sequence that has 95% sequence identity with SEQ ID NO:2 or SEQ ID NO:7, wherein said 5′ untranslated region is inserted between, and operably linked to, said promoter sequence and said transgene.
4 . (canceled)
5 . The nucleic acid expression cassette of claim 1 , further comprising a 3′ untranslated region comprising SEQ ID NO:3 or SEQ ID NO:8, or a sequence that has 95% sequence identity with SEQ ID NO:3 or SEQ ID NO:8, wherein said 3′ untranslated region is operably linked to said transgene.
6 . The nucleic acid expression cassette of claim 5 , wherein said 3′ untranslated region is part of a terminator comprising SEQ ID NO:4 or SEQ ID NO:9, or a sequence that has 95% sequence identity with SEQ ID NO:4 or SEQ ID NO:9.
7 . (canceled)
8 . The nucleic acid expression cassette of claim 1 , wherein said transgene encodes a selectable marker, an interfering RNA, or a gene product conferring insecticidal resistance, herbicide tolerance, nitrogen use efficiency, water use efficiency, or nutritional quality.
9 . The nucleic acid expression cassette of claim 8 , wherein said transgene confers tolerance to an herbicide selected from the group consisting of glyphosate, glufosinate, 2,4-dichlorophenoxyacetate (2,4-D), imidazolinone, sulfonylurea, acetohydroxyacid synthase (AHAS), and acetolactate synthase (ALS).
10 . A nucleic acid vector, comprising a promoter operably linked to (i) a polylinker sequence, (ii) a non-rubisco activase transgene, or (iii) a combination of (i) and (ii), wherein said promoter comprises SEQ ID NO:1 or SEQ ID NO:6, or a sequence that has 95% sequence identity with SEQ ID NO:1 or SEQ ID NO:6.
11 . The nucleic acid vector of claim 10 , further comprising a 5′ untranslated region comprising SEQ ID NO:2 or SEQ ID NO:7, or a sequence that has 95% sequence identity with SEQ ID NO:2 or SEQ ID NO:7, wherein said 5′ untranslated region is inserted between, and operably linked to said promoter sequence and said linker or said transgene.
12 . The nucleic acid vector of claim 10 , further comprising a 3′ untranslated region comprising SEQ ID NO:3 or SEQ ID NO:8, or a sequence that has 95% sequence identity with SEQ ID NO:3 or SEQ ID NO:8, wherein said 3′ untranslated region is operably linked to said linker or said transgene.
13 . The nucleic acid vector of claim 10 , wherein said vector comprises SEQ ID NO:12 or SEQ ID NO:13, or a sequence that has 95% sequence identity with SEQ ID NO:12 or SEQ ID NO:13.
14 . A cell comprising said nucleic acid expression cassette of claim 1 .
15 . (canceled)
16 . A plant or plant part comprising said cell of claim 14 .
17 . The plant of claim 16 , wherein said plant is selected from the group consisting of Arabidopsis , tobacco, tomato, maize, wheat, rice, sorghum, oats, rye, bananas, sugar cane, soybean, cotton, sunflower, and canola.
18 . (canceled)
19 . The cell of claim 14 , further comprising a 5′ untranslated region comprising SEQ ID NO:2 or SEQ ID NO:7, or a sequence that has 95% sequence identity with SEQ ID NO:2 or SEQ ID NO:7, wherein said 5′ untranslated region is inserted between, and operably linked to said promoter sequence and said linker or said transgene.
20 . The cell of claim 14 , further comprising a 3′ untranslated region comprising SEQ ID NO:3 or SEQ ID NO:8, or a sequence that has 95% sequence identity with SEQ ID NO:3 or SEQ ID NO:8, wherein said 3′ untranslated region is operably linked to said linker or said transgene.
21 . A method for expressing a transgene in a plant, comprising growing a plant comprising said gene expression cassette of claim 1 .
22 . The method of claim 21 , wherein said gene expression cassette further comprises a 5′ untranslated region comprising SEQ ID NO:2 or SEQ ID NO:7, or a sequence that has 95% sequence identity with SEQ ID NO:2 or SEQ ID NO:7, wherein said 5′ untranslated region is inserted between, and operably linked to said promoter sequence and said linker or said transgene.
23 . The method of claim 21 , wherein said gene expression cassette further comprises a 3′ untranslated region comprising SEQ ID NO:3 or SEQ ID NO:8, or a sequence that has 95% sequence identity with SEQ ID NO:3 or SEQ ID NO:8, wherein said 3′ untranslated region is operably linked to said linker or said transgene.
24 . A method for expressing a transgene in a plant, comprising transforming a plant with said gene expression cassette of claim 1 .
25 . The method of claim 24 , wherein the gene expression cassette further comprises a 5′ untranslated region comprising SEQ ID NO:2 or SEQ ID NO:7, or a sequence that has 95% sequence identity with SEQ ID NO:2 or SEQ ID NO:7, wherein said 5′ untranslated region is inserted between, and operably linked to said promoter sequence and said linker or said transgene.
26 . The method of claim 24 , wherein the gene expression cassette further comprises a 3′ untranslated region comprising SEQ ID NO:3 or SEQ ID NO:8, or a sequence that has 95% sequence identity with SEQ ID NO:3 or SEQ ID NO:8, wherein said 3′ untranslated region is operably linked to said linker or said transgene.