Plant regulatory elements and methods of use thereof
The present disclosure provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for regulatory elements from Eupatorium Vein Clearing Virus. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory element sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the regulatory elements of the present disclosure.
1. A recombinant nucleic acid molecule comprising a regulatory element operably linked to a heterologous polynucleotide, wherein the regulatory element comprises a polynucleotide selected from the group consisting of:
(a) the polynucleotide of SEQ ID NO: 1, and
(b) a fragment of at least 283 nucleotides of SEQ ID NO: 1; and
and wherein the regulatory element has regulatory activity in a plant on the heterologous polynucleotide.
2. The recombinant nucleic acid molecule of claim 1 , wherein the regulatory element comprises
the polynucleotide of SEQ ID NO: 2.
3. The recombinant nucleic acid molecule of claim 1 , wherein the regulatory element comprises
the polynucleotide of SEQ ID NO: 3.
4. The recombinant nucleic acid molecule of claim 1 , wherein the regulatory element comprises
the polynucleotide of SEQ ID NO: 4.
5. The recombinant nucleic acid molecule of claim 1 , wherein the regulatory element comprises
the polynucleotide of SEQ ID NO: 5.
6. A DNA construct comprising
(a) a regulatory element selected from the group consisting of:
(i) the polynucleotide of SEQ ID NO: 1, and
(ii) a fragment of at least 283 nucleotides of SEQ ID NO: 1; and
(b) a heterologous transcribable polynucleotide molecule operably linked to the regulatory element.
7. The DNA construct of claim 6 , wherein the regulatory element comprises the polynucleotide of SEQ ID NO: 2.
8. The DNA construct of claim 6 , wherein the regulatory element comprises the polynucleotide of SEQ ID NO: 3.
9. The DNA construct of claim 6 , wherein the regulatory element comprises the polynucleotide of SEQ ID NO: 4.
10. The DNA construct of claim 6 , wherein the regulatory element comprises the polynucleotide of SEQ ID NO: 5.
11. The DNA construct of claim 6 , wherein the heterologous transcribable polynucleotide molecule is a gene capable of providing herbicide resistance in plants.
12. The DNA construct of claim 6 , wherein the heterologous transcribable polynucleotide molecule is a gene capable of providing plant pest control in plants.
13. A transgenic plant or plant cell stably transformed with the recombinant polynucleotide of claim 1 .
14. The transgenic plant or plant cell of claim 13 , wherein the transgenic plant is a monocotyledon plant cell.
15. The transgenic plant or plant cell of claim 13 , wherein the transgenic plant is a dicotyledon plant cell.
16. A transgenic plant or plant cell stably transformed with the DNA construct of claim 6 .
17. A plant part of the transgenic plant of claim 15 , wherein the plant part comprises the DNA construct.
18. A seed of the transgenic plant of claim 14 , wherein the seed comprises the DNA construct.