Compositions and methods for controlling plant pests
Novel insecticidal proteins that are toxic to lepidopteran pests are disclosed. The DNA encoding the insecticidal proteins can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. The recombinant organisms or compositions containing the recombinant organisms or the insecticidal proteins alone or in combination with an appropriate agricultural carrier can be used to control lepidopteran pests in various environments.
1. A method of controlling a Cry1Ab-resistant lepidopteran insect comprising delivering to the Cry1Ab-resistant insect an effective amount of an insecticidal protein comprising SEQ ID NO:31.
2. The method of claim 1 , wherein the Cry1Ab-resistant insect is sugarcane borer ( Diatraea saccharalis ).
3. A method of preventing the development of resistance in a population of sugarcane borer ( Diatraea saccharalis ) to a Cry1Ab protein expressed in a transgenic plant comprising stacking in the transgenic plant in addition to the Cry1Ab protein a second insecticidal protein comprising SEQ ID NO:31.