Method and system for propagating a microorganism
Disclosed are methods and systems for propagating microorganisms such as yeast using a carbon source that includes glycerol and a nutrient source that includes a stillage component.
1. A method of propagating a microorganism, the method comprising:
(a) forming a propagation medium, wherein forming comprises combining glycerol and a stillage component, wherein the glycerol is in an amount of from 0.5 to 5 weight percent (wt/v) of the propagation medium, wherein the propagation medium comprises glucose in an amount of from 0 to 0.5 weight percent (wt/v), and wherein the stillage component is whole stillage, thin stillage, wet cake or combinations thereof; and
(b) growing a first cell mass of the microorganism on the propagation medium to propagate the first cell mass of the microorganism to form a second cell mass of the microorganism.
2. The method of claim 1 , wherein the glycerol comprises crude glycerol from a biodiesel production process.
3. The method of claim 1 , wherein the glycerol comprises 75 to 90% pure glycerol.
4. The method of claim 1 , wherein the stillage component comprises an amount from 5 to 35 grams solids per liter of the propagation medium.
5. The method of claim 1 , wherein the stillage component is thin stillage.
6. The method of claim 5 , wherein weight ratio of glycerol to solids of thin stillage is in the range of from 0.05 to 20.
7. The method of claim 1 , wherein the first cell mass of the microorganism comprises an amount of cells less than 5 gram per liter of the propagation medium.
8. The method of claim 1 , wherein the second cell mass of the microorganism comprises an amount of cells from 10 to 30 grams per liter of the propagation medium.
9. The method of claim 1 , wherein the growing is by a batch, fed-batch or continuous process.
10. The method of claim 1 , wherein substantially no ethanol is produced by the first cell mass of the microorganism or second cell mass of the microorganism during the growing for a time period.
11. A system for propagating a microorganism comprising:
(a) a propagation reactor vessel, wherein the propagation reactor vessel contains a propagation medium comprising:
(i) glycerol in an amount of from 0.5 to 5 weight percent (wt/v) of the propagation medium;
(ii) a stillage component, wherein the stillage component comprises whole stillage, thin stillage, wet cake or combinations thereof; and glucose in an amount of from 0 to 0.5 weight percent (wt/v); and
(iii) a first cell mass of microorganism; wherein the microorganism can use the propagation medium as a feed source and wherein the propagation reactor vessel is configured for growth of the first cell mass of microorganism to form a second cell mass of microorganism; and
(b) an aerator coupled to the propagation reactor vessel.