A METHOD AND A SYSTEM FOR DETERMINING A CONCENTRATION RANGE FOR A SAMPLE BY MEANS OF A CALIBRATION CURVE
A system and a method for determining a concentration region for a measurement of a response value by means of a calibration curve is disclosed. The calibration curve includes response values as a function of concentrations. The method includes providing a series of measured calibration data; fitting a regression model to the series of measured calibration data; calculating a standard deviation and a standard error for the measured calibration data; calculating a probability using the t-distribution with parameters including degrees of freedom and a requested confidence interval; calculating a response value interval; applying the response value interval to the calibration model; measuring a response value for a sample; and determining the concentration region by means of the response value interval and the calibration model.
1 . A method for determining a concentration region for a measurement of a response value by means of a calibration curve, the calibration curve comprises response values as a function of concentrations, wherein the method comprising:
providing a series of measured calibration data;
fitting a regression model to the provided series of measured calibration data;
calculating a standard deviation for the measured calibration data;
calculating a standard error for the measured calibration data;
calculating a probability using the t-distribution with parameters, the parameters comprising degrees of freedom, and a requested confidence interval;
calculating a response value interval as a product of the standard error and the probability;
applying the response value interval to the calibration model;
measuring a response value for a sample;
determining the concentration region by means of the response value interval and the calibration model.
2 . The method of claim 1 , wherein the step of applying the response value interval comprises:
calculating a lower curve by means of subtracting the response value interval from the calibration model;
calculating an upper curve by means of adding the response value interval to the calibration model;
calculating the concentration region using the lower curve, the upper curve and a given response value.
3 . The method of claim 1 , wherein the method further comprises:
calculating a first concentration corresponding to a predetermined maximum concentration region;
calculating a second concentration corresponding to a predetermined maximum concentration region, wherein the second concentration region is larger than the first concentration region;
defining a concentration interval from the first concentration to the second concentration.
4 . A computer readable media containing instructions for carrying out the method of claim 1 , when executed by a processor.
5 . A label free interaction analysis system, comprising:
an analysis device;
a control unit comprising a processor and a memory, wherein the memory contains instructions for carrying out the method of claim 1 , when executed by the processor.
6 . A-The label free interaction analysis system of claim 5 , wherein the analysis device is a surface plasmon resonance device.