Therapeutic oligonucleotides
Methods and compositions are provided for oligonucleotides that bind targets of interest. The targets include cells and microvesicles, such as those derived from various diseases. The oligonucleotides can be used for diagnostic and therapeutic purposes. The target of the oligonucleotides can be a target such as PARP1, HIST1H1B, HIST1H1D, NCL, FBL, SFPQ, RPL12, ACTB, HIST1H4A, SSBP1, NONO, H2AFJ, and DDX21, or a complex, subunit or fragment thereof.
1. An oligonucleotide having up to 80 nucleotides and comprising SEQ ID NO. 4357.
2. The oligonucleotide of claim 1 , wherein the oligonucleotide comprises flanking sequences at 5 ′ and/or 3 ′ ends of SEQ ID NO: 4357.
3. The oligonucleotide of claim 1 , wherein the oligonucleotide comprises an oligonucleotide region 5 ′ of SEQ ID NO: 4357, an oligonucleotide region 3 ′ of SEQ ID NO: 4357 or both.
4. The oligonucleotide of claim 1 , wherein the oligonucleotide is capable of binding to cells comprising surface nucleolin.
5. The oligonucleotide of claim 1 , wherein the oligonucleotide is capable of binding to Heterogeneous nuclear ribonucleoprotein U protein (HNRPU), or to a complex comprising HNRPU, or to a cell expressing HNRPU on its surface.
6. A composition comprising the oligonucleotide according to claim 5 .
7. The composition of claim 6 , wherein the oligonucleotide is capable of modulating proliferation of a cell.
8. The composition of claim 6 , wherein the oligonucleotide is capable of inducing apoptosis.
9. The composition of claim 7 , wherein the cell is a leukemia cell, a lymphoma cell or a renal carcinoma cell.
10. A method comprising synthesizing the oligonucleotide according to claim 1 .
11. A method comprising contacting a biological sample with the oligonucleotide according to claim 1 .
12. The method of claim 11 , further comprising detecting a presence or level of a protein represented by a UniProt accession number selected from the group consisting of P19338, P22626, P06748, P60709, P22087, P09651, Q04837, P11142, Q00839, P52272, Q07955, P55769, Q12906, P06576, P31942, Q86V81, Q9NR30, Q96PK6, P37108, P09874, P17096, Q99729, P11021, P23246, P27694, Q13151, P04792, P31943, P51991, P02647, 043390, P08865, 014979, Q15365, P26599, Q14624, Q14103, P04406, 060506, Q15717, Q8NFH5, Q9NX24, P01009, Q14978, P10809, P14866, Q13243, Q6RFH5, Q9NZI8, P46087, P18754, P11940, P25705, Q15173, Q9Y3B9, Q9Y3Y2, P84103, P30154, 000577, 1A31_HUMAN, 000479, P62263, Q16629, P52907, Q12905, P13645, P07910, Q7L8J4, P14923, P16403, P62633, P61978, P35268, Q01844, Q9UKM9, Q14978-1, P62750, P68431, P67809, P50914, Q8TEM1, P46087-4, Q13435, P23246-1, Q9NR30-1, P11387, Q14978-2, P17480-1, 012906-7, Q9NVP1, Q13769, Q08170, P17844, P11940-1, 000567, P05109, Q15233, P06702, Q06830, P25311, P38159-1, P07910-1, P35678, Q05639, 060832-1, Q13601, Q15287-1, P61978-2, Q13247, P42696, Q969G3, P04406-1, P07910-2, Q9UKM9-1, P09651-1, Q08188, P07355-2, P16402, P16401, P62979, Q9BTM1, Q5D862, P30050-1, P31151, 09NY12-1, P62633-1, P15927-3, P62913, P62081, P14678-3, Q9BRL6-1, Q92522, Q07020, P26373-1, Q92979, P09661, Q04387, P62805, P04908, Q5QNW6-2, P53999, P06899, P62888, P62899-2, P61353, P62318, P69905, Q8IUE6, P62829, P62851, P62269, P60866-2, and P49458 in the biological sample that is bound by the oligonucleotide.
13. The method of claim 11 , further comprising detecting a presence or level of a protein represented by a UniProt accession number selected from the group consisting of P09874, P16401, P16402, P19338, P22087, P23246, P30050, P60709, P628-5, Q04837, Q15233, Q9BTM1, and Q9NR30 in Table 61 in the biological sample that is bound by the oligonucleotide.
14. A pharmaceutical composition comprising a therapeutically effective amount of the oligonucleotide according to claim 1 , or a salt thereof, and a pharmaceutically acceptable carrier, diluent, or both.
15. The pharmaceutical composition of claim 14 , wherein the oligonucleotide is attached to a toxin or chemotherapeutic agent.