Affinity support and method for isolating immunoglobulin
To provide an affinity support in which a binding property of a ligand to a target substance is improved. The affinity support contains a solid phase support and a protein ligand, wherein the protein ligand is represented by formula (1): R—R 1 (1) wherein R represents a linker binding to the solid phase support, which contains a polyproline, and R 1 represents a protein showing an affinity to immunoglobulin, and the R is bound to a C terminal or an N terminal of an amino acid sequence in R 1 .
1. An affinity support, comprising:
a solid phase support and
a protein ligand,
wherein the protein ligand has the following formula(1):
R—R 1 (1),
wherein
R is a linker binding to the solid phase support, which comprises a polyproline comprising from 12 to 24 proline residues, and
R 1 is a protein having an affinity to an immunoglobulin, and
R is bound to a C terminal or an N terminal of an amino acid sequence of the protein R 1 .
2. The affinity support according to claim 1 , wherein the linker has a length of from 3.6 nm to 7.3 nm.
3. The affinity support according to claim 1 , wherein the linker comprises an amino acid residue having an amino group or a thiol group at a terminal binding to the solid phase support.
4. The affinity support according to claim 1 , wherein the protein having the affinity to an immunoglobulin comprises an immunoglobulin-binding domain derived from an Fc-binding protein or Protein A.
5. The affinity support according to claim 1 ,
wherein the protein having the affinity to an immunoglobulin comprises at least one immunoglobulin-binding domain selected from the group consisting of
an immunoglobulin-binding domain consisting of the amino acid sequence of SEQ ID NO: 3,
an immunoglobulin-binding domain comprising a partial sequence of the amino acid sequence of SEQ ID NO: 3, and
an immunoglobulin-binding domain consisting of an amino acid sequence having at least 70% identity to the amino acid sequence of SEQ ID NO: 3.
6. The affinity support according to claim 4 , wherein the protein having the affinity to an immunoglobulin comprises two or more of the immunoglobulin-binding domains.
7. The affinity support according to claim 1 , wherein the solid phase support comprises at least one reactive group capable of binding a thiol group or an amino group.
8. The affinity support according to claim 7 , wherein the at least one reactive group is an epoxy group.
9. A method for isolating immunoglobulin, comprising:
isolating the immunoglobulin with the affinity support according to claim 1 .
10. An affinity ligand, comprising:
a linker R and
a protein ligand R 1 ,
wherein
the linker R comprises a polyproline comprising from 12 to 24 proline residues,
R 1 is a protein having an affinity to an immunoglobulin, and
R is bound to a C terminal or an N terminal of an amino acid sequence of the protein R 1 .