Root-preferential and stress inducible promoter and uses thereof
The present invention relates to the field of agriculture. In particular, the invention provides a promoter, a recombinant gene, plants comprising the recombinant genes and a method to improve yield of a cotton plant under stress conditions.
1. A recombinant gene comprising a nucleic acid having root-preferential, stress-inducible or stress-induced root-preferential promoter activity comprising:
a. a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 7 or
b. a nucleic acid comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 7; and further comprising a heterologous sequence.
2. The recombinant gene according to claim 1 , wherein said heterologous nucleic acid sequence encodes an expression product of interest, and optionally a transcription termination and polyadenylation sequence.
3. The recombinant gene according to claim 2 , wherein the expression product of interest is an RNA molecule capable of modulating the expression of a gene or is a protein.
4. A plant cell comprising the recombinant gene according to claim 1 .
5. A plant comprising the recombinant gene of claim 2 .
6. Plant parts and seeds comprising the recombinant gene according to claim 2 .
7. A method of producing a transgenic plant comprising:
regenerating transgenic plants from transgenic cells comprising the recombinant gene according to claim 2 .
8. A method of effecting root-preferential expression of a nucleic acid comprising introducing the recombinant gene according to claim 2 into the genome of a plant.
9. A method of effecting stress-inducible expression of a nucleic acid comprising introducing the recombinant gene according to claim 2 into the genome of a plant.
10. A method of effecting stress-induced expression of a nucleic acid preferentially in the roots comprising introducing the recombinant gene according to claim 2 into the genome of a plant.
11. A method for altering biotic or abiotic stress tolerance, root architecture, nutrient use efficiency, or yield of a plant, said method comprising introducing the recombinant gene according to claim 2 into the genome of a plant.
12. The method according to claim 7 wherein said plant is a cotton, soybean or wheat plant.
13. The recombinant gene of claim 2 , wherein the transcription termination and polyadenylation sequence is functional in plants.
14. A method of effecting root-preferential expression of a nucleic acid comprising providing the plant according to claim 5 .
15. A method of effecting stress-inducible expression of a nucleic acid comprising providing the plant according to claim 5 .
16. A method of effecting stress-inducible expression of a nucleic acid preferentially in the roots comprising providing the plant according to claim 5 .
17. A method for altering biotic or abiotic stress tolerance, root architecture, nutrient use efficiency, or yield of a plant, said method comprising providing the plant according to claim 5 .
18. The method according to claim 8 , wherein said plant is a cotton, soybean or wheat plant.
19. The method according to claim 9 , wherein said plant is a cotton, soybean or wheat plant.
20. The method according to claim 10 , wherein said plant is a cotton, soybean or wheat plant.
21. The recombinant gene of claim 1 , comprising a nucleic acid comprising a nucleotide sequence having at least 98% sequence identity to SEQ ID NO: 7.
22. The recombinant gene of claim 1 , comprising a nucleic acid comprising a nucleotide sequence having at least 99% sequence identity to SEQ ID NO: 7.
23. The recombinant gene of claim 1 comprising the nucleotide sequence of SEQ ID NO: 7.