Substrate delivery of embedded liposomes
This invention relates to compositions useful for localized and sustained release of therapeutic agents, and more particularly to functionalized liposomes embedded in a polyelectrolyte multilayer. Methods of preparing the compositions, methods of treating diseases, devices, and pharmaceutical compositions comprising the compositions are also provided.
1. A composition comprising:
i) one or more hyaluronic acid conjugated liposomes; and
ii) a polyelectrolyte multilayer;
wherein the hyaluronic acid is covalently bonded to the surface of the liposome;
and wherein the one or more hyaluronic acid conjugated liposomes are deposited onto the polyelectrolyte multilayer.
2. The composition of claim 1 , wherein the polyelectrolyte multilayer comprises one or more independently selected polycationic polymers.
3. The composition of claim 1 , wherein the polyelectrolyte multilayer comprises one or more independently selected polyanionic polymers.
4. The composition of claim 3 , wherein at least one of the polyanionic polymers is poly(sodium styrene sulfonate) (SPS).
5. The composition of claim 1 , wherein the polyelectrolyte multilayer comprises from about 2 to about 10 monolayers of poly-L-lysine (PLL) and from about 2 to about 10 monolayers of poly(sodium styrene sulfonate) (SPS).
6. The composition of claim 1 , wherein the liposome comprises a hydrophilic core and a hydrophobic lipid bilayer.
7. The composition of claim 1 , wherein the liposome further comprises one or more therapeutic agents.
8. The composition of claim 6 , wherein each of the therapeutic agents is independently localized in the hydrophilic core or hydrophobic lipid bilayer of the liposome.
9. The composition of claim 1 , wherein the liposome further comprises a fluorescently tagged lipid.
10. The composition of claim 1 , wherein the liposome further comprises a targeting moiety.
11. The composition of claim 10 , wherein the targeting moiety is conjugated to the hyaluronic acid.
12. The composition of claim 1 , further comprising an additional polyelectrolyte multilayer.
13. The composition of claim 1 , further comprising a substrate selected from the group consisting of a medical device, a tablet, a capsule, a bandage, a gel, and a nanoparticle.
14. The composition of claim 2 , wherein at least one of the polycationic polymers is poly-L-lysine (PLL).