Antibodies and assays for detection of CD37
The invention generally relates to antibodies that bind to human CD37 and diagnostic assays for CD37-based therapies.
1. An antibody or antigen-binding fragment thereof that specifically binds to CD37, wherein said antibody or fragment thereof comprises the VH CDR1, CDR2, and CDR3 sequences of SEQ ID NOs: 3-5 and the VL CDR1, CDR2, and CDR3 sequences of SEQ ID NOs: 6-8, respectively.
2. The antibody or antigen-binding fragment thereof of claim 1 , wherein the antibody or fragment thereof comprises the polypeptide sequences of SEQ ID NOs:9 and/or 10.
3. The antibody or antigen-binding fragment thereof of claim 1 , wherein said antibody or antigen-binding fragment thereof is murine, humanized, or chimeric.
4. The antibody or antigen-binding fragment thereof of claim 1 , which is a full length antibody.
5. The antibody or antigen-binding fragment thereof of claim 1 , which is an antigen-binding fragment.
6. The antibody or antigen-binding fragment thereof of claim 5 , wherein said antibody or antigen-binding fragment thereof comprises a Fab, Fab′, F(ab′)2, single chain Fv or scFv, disulfide linked Fv, intrabody, IgGΔCH2, minibody, F(ab′)3, tetrabody, triabody, diabody, DVD-Ig, mAb2, (scFv)2, or scFv-Fc.
7. The antibody or antigen-binding fragment thereof of claim 1 , wherein the antibody or antigen-binding fragment thereof is detectably labeled.
8. A cell producing the antibody or antigen-binding fragment thereof of claim 1 .
9. A method of making the antibody or antigen-binding fragment thereof of claim 1 , comprising (a) culturing a cell producing the antibody or antigen-binding fragment thereof of claim 1 ; and (b) isolating said antibody or antigen-binding fragment thereof from said cultured cell.
10. A composition comprising the antibody or antigen-binding fragment thereof of claim 1 and buffer selected from the group consisting of: an IHC buffer, an ELISA buffer, and a FACS buffer.
11. A method of detecting CD37 expression in a sample comprising contacting said sample with the antibody or antigen-binding fragment thereof of claim 1 .
12. The method of claim 11 , wherein CD37 expression is determined by immunohistochemical (IHC) assay.
13. A method for treating cancer with a therapeutically active agent comprising an anti-CD37 antibody or antigen binding fragment thereof wherein said antibody or antigen binding fragment thereof in the therapeutically active agent is conjugated to a cytotoxin, said method comprising administering the therapeutically active agent to a subject having cancer, wherein increased expression of CD37 has been detected in a cancerous sample from said subject using the antibody or antigen-binding fragment there of claim 1 .
14. The method of claim 13 , wherein the detecting is by immunohistochemistry (IHC).
15. The method of claim 13 , wherein the cancer is a leukemia or a lymphoma.
16. The method of claim 13 , wherein said cancer is selected from the group consisting of B cell lymphomas, NHL, precursor B cell lymphoblastic leukemia/lymphoma and mature B cell neoplasms, B cell chronic lymphocytic leukemia (CLL)/small lymphocytic lymphoma (SLL), B cell prolymphocytic leukemia, lymphoplasmacytic lymphoma, mantle cell lymphoma (MCL), follicular lymphoma (FL), low grade, intermediate-grade and high-grade (FL), cutaneous follicle center lymphoma, marginal zone B cell lymphoma, MALT type marginal zone B cell lymphoma, nodal marginal zone B cell lymphoma, splenic type marginal zone B cell lymphoma, hairy cell leukemia, diffuse large B cell lymphoma, Burkitt's lymphoma, plasmacytoma, plasma cell myeloma, post-transplant lymphoproliferative disorder, Waldenstrom's macroglobulinemia, and anaplastic large-cell lymphoma (ALCL).
17. The method of claim 13 , wherein the therapeutically active agent is an antibody maytansinoid conjugate that comprises a CD37 antibody comprising a heavy chain variable region comprising SEQ ID NO:28 and a light chain variable region comprising SEQ ID NO:29.
18. The method of claim 17 , wherein the therapeutically active agent comprises the maytansinoid DM1, the non-cleavable SMCC linker, and wherein the CD37 antibody comprises a full length heavy chain comprising SEQ ID NO:30 and a full length light chain comprising SEQ ID NO:31.
19. The method of claim 16 , wherein said cancer is diffuse large B cell lymphoma.