Decellularized biomaterial and method for formation
Methods for developing a decellularized tissue and biomaterials for use as biomimetic grafts or in vitro cellular scaffolds formed with the decellularized tissue are described. The biomaterials are particularly well suited for use as an intervertebral disc graft. The decellularized tissue is formed from an intervertebral disc source tissue and can be substantially decellularized and substantially free of potential immunogenic material (e.g., DNA and RNA), while maintaining ECM materials including both glycosaminoglycan and collagen.
1. A biomaterial comprising a decellularized bovine intervertebral disc tissue, the decellularized bovine intervertebral disc tissue being substantially free of cell nuclei of a source bovine intervertebral disc tissue, the decellularized bovine intervertebral disc tissue comprising decellularized nucleus pulposus tissue, the decellularized nucleus pulposus tissue having a glycosaminoglycan content of about 200 micrograms or greater per milligram dry weight of the decellularized nucleus pulposus tissue and having a nucleic acid content of about 50 nanograms or less per milligram dry weight of the decellularized nucleus pulposus tissue.
2. The biomaterial of claim 1 , the decellularized intervertebral disc tissue having a ratio of glycosaminoglycan content to hydroxyproline content of from about 10:1 to about 25:1.
3. The biomaterial of claim 1 , further comprising extrinsic cells.
4. The biomaterial of claim 3 , wherein the extrinsic cells comprise stem cells.
5. The biomaterial of claim 1 , wherein one or more components of the decellularized intervertebral disc tissue is crosslinked.
6. The biomaterial of claim 1 , wherein the biomaterial is an implantable graft material.
7. The biomaterial of claim 6 , wherein the implantable graft material is an implantable intervertebral disc graft.
8. The biomaterial of claim 1 , wherein the biomaterial is an in vitro cellular scaffold material.
9. The biomaterial of claim 1 , further comprising annulus fibrosus tissue.
10. The biomaterial of claim 1 , wherein the biomaterial comprises an entire intervertebral disc.