Micro-needle sampling device and use thereof
View Patent ↗The present invention relates to a device ( 10 ) for obtaining a sample ( 30 ) from a biological material ( 40 ) in solid form, said device comprising an array of micro-needles ( 30 ) arranged on a base plate ( 20 ). It further relates to a method for obtaining a sample ( 50 ) from a biological material ( 40 ) in solid form, comprising pressing the micro-needles ( 30 ) of the device ( 10 ) into said biological material ( 40 ), and subsequently removing the device from the biological material ( 40 ), and to the use of the device ( 10 ) in such a method.
1. A method for obtaining a sample from a biological material in solid form for storage on a solid medium, comprising pressing micro-needles of a device into said biological material, characterized in said device comprising an array of micro-needles arranged on a base plate, wherein the micro-needles are coated with a coating enhancing adherence of biological material to the micro-needles, wherein said coating is selected from extracellular matrix attachment and/or adhesion proteins, mucopolysaccharides, basic synthetic polymers, or any combination thereof, and subsequently removing the device from the biological material and transferring the sample of the biological material to said solid medium by pressing the micro-needles into the solid medium.
2. The method according to claim 1 , wherein the micro-needles are solid.
3. The method according to claim 1 , wherein the micro-needles have a length of 0. 1-1.5 mm.
4. The method according to claim 1 , wherein the micro-needles have an average diameter of 0.1-0.3 mm.
5. The method according to claim 1 , wherein the micro-needles are in the shape of cones, pyramids, or rods with conical or pyramidal tips, pointing upward from the base plate.
6. The method according to claim 1 , wherein the micro-needles have a barbed or rugged surface.
7. The method according to claim 1 , wherein the coating comprises extracellular matrix attachment and/or adhesion proteins selected from collagen, laminin and fibronectin; mucopolysaccharides selected from heparin sulfate, hyaluronidate and chondroitin sulfate; basic synthetic polymers selected from poly-D-lysine; or any combination thereof.
8. The method according to claim 1 , wherein the concentration of the micro-needles on the base plate is 400-12,000 micro-needles per cm 2 .
9. The method according to claim 1 , wherein the base plate is made of a flexible material.
10. The method according to claim 1 , wherein the micro-needles and the base plate are made of the same or different material.
11. The method according to claim 1 , wherein the micro-needles and/or the base plate are made of a material selected from silica, epoxy resins, acrylic polymers, polyurethane, polypropylene, silicone resins, ceramics, metal, or a combination thereof.