Granted Patent B2

US 12,109,274 · App. 15/760,686 · Granted Oct 8, 2024

Inventors: Gabor Butora (Cambridge, MA); Andrew W. Fraley (Arlington, MA); Edward John Miracco (Cambridge, MA); Jennifer Nelson (Brookline, MA); Amy Rhoden Smith (Watertown, MA); Matthew Stanton (Marlton, NJ); Brian R. Fritz (Cambridge, MA)

Assignee: ModernaTX, Inc.

A61K48/0066C07F9/65586C07F9/65616C07H21/02C12N15/67

Abstract

Claims (17)

1. A polynucleotide encoding a polypeptide, wherein said polynucleotide comprises:

(a) a 5′-cap structure;

(b) a 5′-UTR;

(c) a coding region encoding the polypeptide;

(d) a 3′-UTR; and

(e) a 3′-stabilizing region comprising 1 to 500 nucleosides, wherein one or more of said nucleosides is an inverted thymidine.

2. The polynucleotide of claim 1 , wherein said polynucleotide further comprises (f) a poly-A region.

3. The polynucleotide of claim 1 , wherein said stabilizing region comprises at least two different nucleosides.

4. The polynucleotide of claim 1 , wherein said stabilizing region comprises 10 nucleosides.

5. The polynucleotide of claim 1 , wherein the 3′-stabilizing region comprises a 5′-terminus, wherein said 5′-terminus of said 3′-stabilizing region is conjugated to the 3′-terminus of said poly-A region.

6. The polynucleotide of claim 1 , wherein said polynucleotide comprises a 3′-terminus, wherein said 3′-stabilizing region comprises said 3′-terminus of said polynucleotide.

7. The polynucleotide of claim 2 , wherein said poly-A region comprises at least one alternative nucleoside.

8. The polynucleotide of claim 3 , wherein at least one nucleoside is 2′-O-methyl-adenosine.

9. The polynucleotide of claim 8 , wherein said stabilizing region comprises the structure:

or a salt thereof;

wherein each X is, independently O or S; and

A represents adenine and T represents thymine.

Assignments (7)