Polypeptides capable of producing glucans having alpha-1,2 branches and use of the same
Disclosed herein are proteins capable of forming glucans having alpha-1,2 linkages/branches, reactions and methods for producing such glucan, compositions comprising such glucan, and various applications thereof.
1. A reaction composition comprising at least water, sucrose, an alpha-glucan substrate, and a polypeptide that is capable of forming at least one alpha-1,2 branch from the alpha-glucan substrate, wherein the polypeptide comprises an amino acid sequence that is at least 90% identical to:
(i) the mature form of SEQ ID NO:4,
(ii) positions 36 to 1115 of SEQ ID NO:4, or
(iii) SEQ ID NO:4.
2. The reaction composition of claim 1 , wherein the polypeptide comprises an amino acid sequence that is at least 90% identical to the mature form of SEQ ID NO:4, wherein said mature form is represented by positions 36 to 1672 of SEQ ID NO:4.
3. The reaction composition of claim 1 , wherein the polypeptide comprises an amino acid sequence that is at least 90% identical to positions 36 to 1115 of SEQ ID NO:4.
4. The reaction composition of claim 1 , wherein the alpha-glucan substrate has a degree of polymerization of at least 3, and comprises at least (i) alpha-1,6 glycosidic linkages or (ii) alpha-1,6 and alpha-1,3 glycosidic linkages.
5. A method of producing a glucan composition that comprises alpha-1,2 linkages, the method comprising:
(a) providing at least the following reaction components: water, sucrose, an alpha-glucan substrate, and a polypeptide that is capable of forming at least one alpha-1,2 branch from the alpha-glucan substrate, wherein said polypeptide comprises an amino acid sequence that is at least 90% identical to:
(i) the mature form of SEQ ID NO:4,
(ii) positions 36 to 1115 of SEQ ID NO:4, or
(iii) SEQ ID NO:4; and
(b) combining the reaction components under suitable conditions whereby the polypeptide catalyzes the synthesis of alpha-1,2 branches from the alpha-glucan substrate, thereby forming a glucan composition comprising alpha-1,2 linkages.
6. The method of claim 5 , wherein the alpha-glucan substrate comprises at least (i) alpha-1,6 glycosidic linkages or (ii) alpha-1,6 and alpha-1,3 glycosidic linkages.
7. The method of claim 5 , wherein the alpha-glucan substrate and sucrose are present in step (b) at a ratio between 0.01:1 and 1:0.01, inclusive.
8. The method of claim 5 , wherein the reaction components further comprise an alpha-glucanohydrolase.
9. A composition that comprises a glucan composition comprising alpha-1,2 linkages produced by the method of claim 5 .
10. The composition of claim 9 , wherein the glucan composition is water-soluble.
11. The composition of claim 9 , wherein the composition is a personal care product.
12. The composition of claim 9 , wherein the composition is a household care product.
13. The composition of claim 9 , wherein the composition is an industrial product.
14. The composition of claim 9 , wherein the composition is a detergent composition.
15. The reaction composition of claim 1 , wherein the alpha-glucan substrate comprises at least 70% alpha-1,6 linkages.
16. The reaction composition of claim 15 , wherein the alpha-glucan substrate comprises at least 80% alpha-1,6 linkages.
17. The reaction composition of claim 16 , wherein the alpha-glucan substrate comprises at least 95% alpha-1,6 linkages.
18. The reaction composition of claim 1 , wherein the alpha-glucan substrate has a degree of polymerization of 8 to 500.
19. The reaction composition of claim 1 , wherein the alpha-glucan substrate has a degree of polymerization of at least 50.
20. The reaction composition of claim 2 , wherein the polypeptide comprises an amino acid sequence that is at least 95% identical to the mature form of SEQ ID NO:4.
21. The reaction composition of claim 3 , wherein the polypeptide comprises an amino add sequence that is at least 95% identical to positions 36 to 1115 of SEQ ID NO:4.
22. The reaction composition of claim 17 , wherein the alpha-glucan substrate comprises 100% alpha-1,6 linkages.
23. The reaction composition of claim 1 , wherein the alpha-glucan substrate has a degree of polymerization of 10 to 2000.
24. The reaction composition of claim 1 , wherein the alpha-glucan substrate has a degree of polymerization of 15 to 2000.
25. The reaction composition of claim 1 , wherein the alpha-glucan substrate has a degree of polymerization of 10 to 1300.
26. The reaction composition of claim 1 , wherein the alpha-glucan substrate has a degree of polymerization of 15 to 1300.
27. The reaction composition of claim 1 , wherein the alpha-glucan substrate has a degree of polymerization of 10 to 150.
28. The reaction composition of claim 1 , wherein the alpha-glucan substrate has a degree of polymerization of 15 to 150.
29. The method of claim 5 , further comprising:
(c) isolating the glucan composition comprising alpha-1,2 linkages.
30. The method of claim 5 , wherein the polypeptide comprises an amino acid sequence that is at least 90% identical to the mature form of SEQ ID NO:4, wherein said mature form is represented by positions 36 to 1672 of SEQ ID NO:4.
31. The method of claim 30 , wherein the polypeptide comprises an amino acid sequence that is at least 95% identical to the mature form of SEQ ID NO:4.
32. The method of claim 5 , wherein the polypeptide comprises an amino acid sequence that is at least 90% identical to positions 36 to 1115 of SEQ ID NO:4.
33. The method of claim 32 , wherein the polypeptide comprises an amino acid sequence that is at least 95% identical to positions 36 to 1115 of SEQ ID NO:4.