IP Library › Granted Patent US 11,014,069
Granted Patent B2
US 11,014,069 · App. 15/769,400 · Granted May 25, 2021

Method and chromatography medium

Inventors: Nils Norrman (Uppsala, SE); Mattias Algotsson (Uppsala, SE); Stefan D. Eriksson (Uppsala, SE)
Assignee: Cytiva BioProcess R&D AB
B01J20/24B01J20/265B01J20/267B01J20/28016B01J20/29B01J20/305B01J20/3208B01J20/3214B01J20/3225B01J20/3242B01J20/3295B01D15/361B01D15/3804B01D15/3847
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Quick Facts
Patent No.
US 11,014,069
App. No.
15/769,400
Granted
May 25, 2021
Kind
B2
Abstract

The present invention relates to a method to improve chromatography beads. More closely, the invention relates to a novel method for production of dextran-containing porous media and chromatography media produced with this method. In the method, the chromatography media is subjected to dextranase-treatment leading to improved pressure-flow properties of the media.

Claims (8)

1. A method to produce a chromatography medium, the chromatography medium comprising porous beads coupled with dextran inside the pores and on the bead, the method comprising subjecting the chromatography medium to dextranase treatment wherein the dextranase is coupled to a magnetic support particle.

2. The method according to claim 1 , wherein the magnetic support particle is larger than the pores of the chromatography media to be treated.

3. The method according to claim 1 , wherein the magnetic support particle comprises natural or synthetic chromatography beads or PEG (poly ethylene glycol).

4. The method according to claim 3 , wherein the dextranase-coupled support particle enters the pores of the chromatography medium.

5. The method according to claim 1 , wherein the chromatography medium is made of natural or synthetic resins.

6. The method according to claim 5 , wherein the chromatography medium is made of agarose.

7. The method according to claim 6 , wherein the agarose is cross-linked.

8. The method according to claim 1 , wherein the dextran coupled to the chromatography medium is provided with a ligand.

Assignments (2)
CHANGE OF NAME Recorded Oct 5, 2020
From: GE HEALTHCARE BIOPROCESS R&D AB
To: CYTIVA BIOPROCESS R&D AB
Reel/Frame 054299/0349 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 19, 2018
From: NORRMAN, NILS; ALGOTSSON, MATTIAS; ERIKSSON, STEFAN D.
To: GE HEALTHCARE BIOPROCESS R&D AB
Reel/Frame 045584/0746 →