Plasma compositions and methods of use thereof

1. A method for preparing pathogen-inactivated cryo-poor plasma, the method comprising:

a) photochemically inactivating one or more units of plasma in the presence of a psoralen, wherein the photochemical inactivation is performed under sterile conditions in a first container containing the one or more units of plasma;

b) transferring under sterile conditions the one or more units of plasma from the first container to a compound absorption device (CAD) coupled to the first container;

c) transferring under sterile conditions the one or more units of plasma from the CAD to two or more second containers coupled to the CAD to provide pathogen-inactivated plasma;

d) transferring under sterile conditions the pathogen-inactivated plasma from the two or more second containers to a third container coupled to the two or more second containers;

e) freezing the pathogen-inactivated plasma followed by thawing of the pathogen-inactivated plasma under conditions that provide for the formation of a cryoprecipitate and pathogen-inactivated cryo-poor plasma;

f) transferring the pathogen-inactivated cryo-poor plasma to at least a first of the two or more second containers; and

g) transferring the cryoprecipitate to a second of the two or more second containers.

2. The method of claim 1 , wherein at least a portion of the pathogen-inactivated cryo-poor plasma is transferred to each of at least two second containers in step f), and wherein the cryoprecipitate is transferred to a third second container in step g).

3. The method of claim 1 , wherein step c) comprises transferring the one or more units of plasma from the CAD to three second containers coupled to the CAD to provide pathogen-inactivated plasma.

4. The method of claim 1 , wherein the third container is coupled to the two or more second containers by sterile docking to tubing between the CAD and the two or more second containers.

5. The method of claim 1 , wherein the one or more units of plasma comprise one or more plasma units from a whole blood donation.

6. The method of claim 1 , wherein the one or more units of plasma comprise one or more plasma units from apheresis collected plasma.

7. The method of claim 1 , wherein the one or more units of plasma comprise plasma pooled from multiple units.

8. The method of claim 1 , wherein the psoralen is amotosalen.

9. The method of claim 1 , wherein the pathogen-inactivated cryo-poor plasma is prepared from at least about 550 mL and less than about 650 mL of pathogen-inactivated plasma.

10. The method of claim 1 , wherein, prior to step g), the cryoprecipitate is resuspended in about 80 mL to about 120 mL of pathogen-inactivated cryo-poor plasma.

11. A method for infusing pathogen-inactivated cryo-poor plasma into a subject, comprising infusing into a subject in need thereof a therapeutically effective amount of a pathogen-inactivated cryo-poor plasma prepared by the method of claim 1 .

12. The method of claim 11 , wherein the subject is suffering from one or more of burns, blunt trauma, penetrating trauma, and hemorrhage.

13. The method of claim 11 , wherein the infusion results in fluid resuscitation of the subject.

14. The method of claim 11 , wherein the pathogen-inactivated cryo-poor plasma is infused into the subject by therapeutic plasma exchange.

15. The method of claim 11 , further comprising, prior to the infusion:

1) freezing the pathogen-inactivated cryo-poor plasma; and

2) thawing the pathogen-inactivated cryo-poor plasma.

16. The method of claim 15 , wherein the pathogen-inactivated cryo-poor plasma is infused into the subject within 5 days after thawing.