Detection device, detection method, and detection system
In the present invention, a detection device has a chip holder, an excitation-light radiation unit, and a heat source. The chip holder is used for positioning a detection chip that has: a prism; a metal film; a trapping body; and a substrate. The excitation-light radiation unit radiates excitation light at the reaction site via the light-entry surface of the detection chip, which is held by the chip holder. The heat source is disposed in a position so as to face, in a non-contacting manner, the surface of the prism of the detection chip held by the chip holder that is closest to the reaction site, and so as not to interfere with the optical path of the excitation light. The chip holder positions the detection chip while in contact with only the reverse surface of the substrate and/or a convex portion positioned on the prism.
1. A detection apparatus for detecting presence or an amount of a detection object substance contained in a sample by utilizing surface plasmon resonance, the detection apparatus comprising:
a detection chip including a prism including an incidence surface, an emission surface and a film formation surface, a metal film formed on the film formation surface, a capturing body disposed in a reaction site that is at least a part of a region on the metal film, and a base that forms, together with the metal film or the film formation surface, a liquid storage that stores liquid;
a chip holder for positioning the detection chip;
an excitation light irradiator that emits excitation light toward a rear surface of the metal film through the incidence surface of the detection chip held in the chip holder, the rear surface corresponding to the reaction site;
a heat source disposed to face one surface of the prism of the detection chip held in the chip holder without making contact with the one surface of the prism, the one surface of the prism being closest to the reaction site among the surfaces of the prism which do not interfere with a light path of the excitation light; and
a light detector that detects fluorescence generated by a fluorescence material labelling the detection object substance contained in the sample captured by the capturing body in the reaction site and excited by the excitation light, or detects reflection light of excitation light reflected by the metal film and emitted from the emission surface utilizing surface plasmon resonance, wherein
the chip holder positions the detection chip by making contact with at least one of a bottom surface of the base and a reference surface disposed on the prism, or by holding at least one of the bottom surface and the reference surface, and
the heat source does not contact the bottom surface of the base.
2. The detection apparatus according to claim 1 , wherein, when the detection chip is held in the chip holder, a distance between the heat source and the one surface closest to the reaction site is equal to or smaller than 3.5 mm.
3. The detection apparatus according to claim 1 , wherein, when the detection chip is held in the chip holder, a distance between the heat source and the one surface closest to the reaction site is equal to or smaller than 0.5 mm.
4. The detection apparatus according to claim 1 , wherein, when the detection chip is held in the chip holder, a distance between the reaction site and a contacting part of the chip holder and the detection chip is greater than a distance between the heat source and the reaction site.
5. The detection apparatus according to claim 1 , wherein the chip holder includes a heat insulating member that blocks or reduces heat conduction from the heat source to the detection chip when the detection chip is held in the chip holder.
6. The detection apparatus according to claim 1 , wherein the chip holder has a bottom wall, and the heat source is on the bottom wall.
7. A method of detecting presence or an amount of a detection object substance contained in a sample by utilizing surface plasmon resonance, the method comprising:
preparing a detection chip including a prism including an incidence surface, an emission surface and a film formation surface, a metal film formed on the film formation surface, a capturing body disposed in a reaction site that is at least a part of a region on the metal film, and a base that forms, together with the metal film or the film formation surface, a liquid storage that stores liquid, the detection chip including a reference surface formed at a bottom surface of the base or the prism;
positioning the detection chip in a chip holder by bringing at least one of the bottom surface and the reference surface into contact with the chip holder, or by holding at least one of the bottom surface and the reference surface on the chip holder;
heating liquid in the liquid storage with a heat source, the heat source being disposed to face one surface of the prism of the detection chip positioned in the chip holder without making contact with of the one surface of the prism, the one surface of the prism being closest to the reaction site among the surfaces of the prism which do not interfere with a light path of excitation light emitted toward a rear surface of the metal film through the incidence surface, the rear surface corresponding to the reaction site;
emitting excitation light toward the rear surface of the metal film that corresponds to the reaction site through the incidence surface; and
detecting fluorescence generated by a fluorescence material labelling the detection object substance contained in the sample captured by the capturing body in the reaction site and excited with the excitation light, or reflection light of excitation light reflected by the metal film and emitted from the emission surface utilizing surface plasmon resonance,
wherein the heat source does not contact the bottom surface of the base.
8. A detection system for detecting presence or an amount of a detection object substance contained in a sample by utilizing surface plasmon resonance, the detection system comprising:
a detection chip including a prism including an incidence surface, an emission surface and a film formation surface, a metal film formed on the film formation surface, a capturing body disposed in a reaction site that is at least a part of a region on the metal film, and a base that forms, together with the metal film or the film formation surface, a liquid storage that stores liquid, the detection chip including a reference surface formed at a bottom surface of the base and the prism;
a chip holder for positioning the detection chip by making contact with at least one of the bottom surface and the reference surface, or by holding the bottom surface and the reference surface;
an excitation light irradiator that emits excitation light toward the reaction site through the incidence surface;
a heat source disposed to face one surface of the prism of the detection chip held in the chip holder without making contact with the one surface of the prism, the one surface of the prism being closest to the reaction site among the surfaces of the prism which do not interfere with a light path of the excitation light; and
a light detector that detects fluorescence generated by a fluorescence material labelling the detection object substance contained in the sample captured by the capturing body in the reaction site and excited by the excitation light, or detects reflection light of excitation light reflected by the metal film and emitted from the emission surface utilizing surface plasmon resonance,
wherein the heat source does not contact the bottom surface of the base.