Method for manufacturing porous particles, porous particles, carrier, column, and method for separating target substance
To provide a porous particle with which non-specific adsorption is hardly generated although the porous particle is a synthetic polymer-based particle, the mechanical strength is high, and the dynamic binding capacity is high in a case where a ligand is bound to the porous particle; and a method for producing the same. A method for producing a porous particle, including the following steps 1 and 2; (step 1) dissolving at least one or more of polymers selected from the group consisting of a vinyl alcohol polymer and an ethylene-vinyl alcohol copolymer in an aqueous solvent to prepare a polymer solution; and (step 2) dispersing the polymer solution in a non-aqueous solvent to form a W/O emulsion.
1. A method for producing a porous particle, the method comprising:
(1) dissolving at least one polymer selected from the group consisting of a vinyl alcohol polymer and an ethylene-vinyl alcohol copolymer in an aqueous solvent to prepare a polymer solution;
(2) dispersing the polymer solution in a non-aqueous solvent to form a W/O emulsion;
(3) cooling the W/O emulsion to form a porous particle;
(4) chemically crosslinking the porous particle by reacting the porous particle with an aldehyde crosslinking agent to obtain a chemically crosslinked porous particle containing a formyl group;
(5) contacting the chemically crosslinked porous particle containing a formyl group with at least one polymer selected from the group consisting of a vinyl alcohol polymer and an ethylene-vinyl alcohol copolymer in the presence of an acid catalyst; and
(6) introducing a reactive functional group capable of binding to a ligand into the porous particle, wherein the reactive functional group is an epoxy compound;
wherein in step (5) the formyl group of the chemically crosslinked porous particle reacts with a hydroxyl group of the at least one polymer and chemically blocks the formyl group.
2. The method according to claim 1 , wherein the at least one polymer comprises an ethylene-vinyl alcohol copolymer having a repeating unit derived from ethylene represented by Formula (1) in an amount exceeding 0% by mole and 50% by mole or less relative to an entire repeating unit in the ethylene-vinyl alcohol copolymer:
CH 2 —CH 2 (1)
3. The method according to claim 1 , wherein the aqueous solvent is a mixed solvent comprising:
at least one selected from the group consisting of a solvent miscible with water among sulfoxide-based solvents, a solvent miscible with water among polyol-based solvents, a solvent miscible with water among alcohol-based solvents, and a solvent miscible with water among amide-based solvents, and water; or
water.
4. The Method according to claim 1 , wherein said contacting a chemically crosslinked porous particle in step (5) is with an ethylene-vinyl alcohol copolymer.
5. The method according to claim 1 , wherein said contacting a chemically crosslinked porous particle in step (5) is with a vinyl alcohol copolymer.
6. The method according to claim 1 , wherein the chemical crosslinking of step (4) is conducted in the presence of an acid catalyst.
7. The method according to claim 6 , wherein the acid catalyst employed is the same acid catalyst present in the contacting of step (5).
8. The method according to claim 7 , wherein the acid catalyst is sulfuric acid.