Plant regulatory elements and methods of use thereof
The present disclosure relates to the field of plant molecular biology, more particularly to regulation of gene expression in plants.
1. A DNA construct comprising a heterologous transcribable polynucleotide molecule operably linked to a regulatory element polynucleotide, wherein the regulatory element polynucleotide is selected from the group consisting of:
(a) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 7; and
(b) a fragment of at least 100 contiguous nucleotides of SEQ ID NO: 7 having regulatory activity.
2. The DNA construct of claim 1 , further comprising a polynucleotide sequence selected from: SEQ ID NO: 1, 2, and 3.
3. A heterologous cell stably transformed with the DNA construct of claim 1 .
4. A transgenic plant or plant cell stably transformed with the DNA construct of claim 1 .
5. The transgenic plant or plant cell of claim 4 , wherein the transgenic plant is a dicotyledon plant cell.
6. The transgenic plant or plant cell of claim 4 , wherein the transgenic plant is a monocotyledon plant cell.
7. A seed of the transgenic plant of claim 4 , wherein the seed comprises the DNA construct.
8. A method for expressing a polynucleotide in a plant, said method comprising introducing into a plant cell a recombinant polynucleotide, said recombinant polynucleotide comprising a regulatory element, wherein said regulatory element comprises a nucleotide sequence selected from the group consisting of:
a. a nucleotide sequence comprising the nucleotide sequence of SEQ ID NO: 7; and
b. a nucleotide sequence comprising a fragment of at least 100 contiguous nucleotides of the nucleotide sequence of SEQ ID NO: 7, wherein the sequence has regulatory activity in a plant cell; and
wherein the sequence has regulatory activity in a plant cell.
9. The method of claim 8 , wherein said plant is a dicot.
10. The method of claim 8 , wherein said plant is a monocot.