Affinity cell extraction by acoustics
Beads with functionalized material applied to them are exposed to an acoustic field to trap or pass the beads. The beads may include or be free of ferro magnetic material. The beads may be biocompatible or biodegradable for a host. The size of the beads may vary over a range, and/or be heterogenous or homogenous. The composition of the beads may include high, neutral or low acoustic contrast material. The chemistry of the functionalized material may be compatible with existing processes.
1. A method for separating material in a fluid, comprising:
applying a functionalized material to a plurality of non-magnetic biodegradable agarose beads that are acoustically responsive;
in the fluid, exposing the beads to biomaterial with an affinity for the functionalized material to permit the biomaterial to bind to the beads, wherein the biomaterial is T cells; and
exposing the beads in the fluid to an acoustic field and trapping the beads in the acoustic field.
2. The method of claim 1 , wherein the functionalized material is a streptavidin conjugate or a biotin conjugate.
3. The method of claim 1 , wherein the functionalized material is composed with an affinity for one or more of CD3, CD4 or CD8 receptors.
4. The method of claim 1 , wherein the functionalized material includes affine molecules that are one or more of antibodies, aptamers or oligonucleotides.
5. The method of claim 1 , wherein the functionalized material comprises a ligand.
6. The method of claim 1 , further comprising reversing a binding between the biomaterial and the beads.
7. The method of claim 1 , further comprising:
a first biomaterial and a distinct second biomaterial included in the biomaterial;
isolating the first biomaterial and isolating the second biomaterial; and
combining the first biomaterial and the second biomaterial in a predetermined ratio.
8. The method of claim 1 , further comprising:
targeted material and other material included in the biomaterial; and
providing beads with an affinity for the targeted material to the fluid.
9. The method of claim 8 , further comprising increasing a density of the fluid to modify an acoustic contrast between the T cells and the fluid.
10. The method of claim 1 , wherein the plurality of beads include at least two different sizes of beads, each size being configured with an affinity for a different type of biomaterial.
11. A method for separating material in a fluid, comprising:
generating an acoustic standing wave in a chamber suitable for housing a fluid;
providing non-magnetic bead-biomaterial complexes to the chamber, wherein the beads are acoustically responsive non-magnetic biodegradable agarose beads, the biomaterial is T cells and the T cells are bound to the beads via a functionalized material with an affinity for the T cells; and
in the fluid, trapping the non-magnetic bead-biomaterial complexes in the acoustic standing wave.
12. The method of claim 11 , wherein the acoustic standing wave is a multidimensional acoustic standing wave.
13. The method of claim 11 , further comprising binding the biomaterial to the non-magnetic beads to form the bead-biomaterial complexes prior to being provided to the chamber.
14. The method of claim 11 , further comprising trapping greater than 90% of the non-magnetic bead-biomaterial complexes in the acoustic standing wave.