Method for reducing the inflammatory activity of a stem cell transplant and use thereof
The disclosure is in the field of cell therapy, more in particular, stem cell transplantation therapy. The disclosure provides methods and compositions for improving the efficacy of stem cell transplantation therapy by reducing the inflammatory activity of a stem cell transplant. More in particular, the disclosure provides a method for preparing a stem cell transplant with reduced inflammatory activity comprising a step of suspending a composition comprising stem cells in a fibrinogen-depleted plasma and/or in a fibrinogen and C-reactive protein-depleted plasma.
1. A composition comprising (i) a hematopoietic stem cell and (ii) plasma or serum, wherein the plasma or serum is depleted of fibrinogen and C-reactive protein.
2. The composition of claim 1 , wherein, additionally, the composition is depleted of pro-inflammatory cells.
3. The composition of claim 1 , wherein the hematopoietic stem cell is a human hematopoietic stem cell.
4. A method of treating a subject to induce tissue regeneration in a subject in need thereof, wherein the tissue regeneration is selected from the group consisting of neural, vascular and hematopoietic tissue regeneration, comprising administering to the subject a therapeutically effective amount of the composition of claim 1 .
5. The method according to claim 4 , wherein the tissue regeneration is a therapy for spinal cord injury.
6. The method according to claim 4 , wherein the stem cell is autologous.
7. The method of claim 6 , wherein the treatment is autologous transplantation therapy.