Microscope including a variable-focus optical system disposed between a photodetector and an objective optical system
To provide a microscope including: an objective optical system that condenses light from a sample; a relay optical system that relays the light condensed by the objective optical system; a photodetector detects the light coming from the objective optical system and relayed by the relay optical system; and a variable-focus optical system disposed at a position between the photodetector and the objective optical system, the position being optically conjugate to a pupil of the objective optical system, the variable-focus optical system being capable of changing a focal position of the objective optical system in a direction along an objective optical axis.
1. A microscope comprising:
an objective optical system that condenses light from a sample;
a relay optical system that relays the light condensed by the objective optical system;
a photodetector that detects the light coming from the objective optical system and relayed by the relay optical system;
a variable-focus optical system disposed at a position between the photodetector and the objective optical system, the position being optically conjugate to a pupil of the objective optical system, and the variable-focus optical system being capable of changing a focal position of the objective optical system in a direction along an objective optical axis;
a microscope body; and
an inner focus unit detachably attached to the microscope body, the inner focus unit having the objective optical system attached to a tip thereof,
wherein:
the inner focus unit includes the relay optical system and the variable-focus optical system,
the microscope body includes a revolver attaching unit for detachably attaching a revolver, the revolver variably retaining a plurality of objective lenses, and
the inner focus unit is detachably attached to the revolver attaching unit, and the variable-focus optical system is provided at a position located at a pupil position of an objective lens retained by the revolver when the revolver is attached to the revolver attaching unit.
2. The microscope according to claim 1 , wherein the inner focus unit includes one of more rotation mechanisms that change at least one of a position and a direction of the objective optical axis of the objective optical system attached to the tip thereof.
3. The microscope according to claim 2 , wherein the microscope is a gate-type microscope.
4. The microscope according to claim 1 , wherein:
the variable-focus optical system is an electrically tunable lens (ETL), and
the ETL is disposed so as to maintain an optical axis direction of the ETL in a vertical direction.
5. The microscope according to claim 1 , wherein the microscope is a two-photon laser scanning microscope, a laser scanning microscope, or a disk scanning confocal microscope.
6. A microscope comprising:
an objective optical system that condenses light from a sample;
a relay optical system that relays the light condensed by the objective optical system;
a photodetector that detects the light coming from the objective optical system and relayed by the relay optical system;
a variable-focus optical system disposed at a position between the photodetector and the objective optical system, the position being optically conjugate to a pupil of the objective optical system, and the variable-focus optical system being capable of changing a focal position of the objective optical system in a direction along an objective optical axis;
a microscope body; and
an inner focus unit detachably attached to the microscope body, the inner focus unit having the objective optical system attached to a tip thereof,
wherein:
the inner focus unit includes the relay optical system and the variable-focus optical system,
the microscope body includes a revolver that variably and detachably retains a plurality of objective lenses, and
the inner focus unit is detachably attached to an objective lens attaching unit of the revolver, and the variable-focus optical system is provided at a position located at a pupil position of an objective lens retained by the revolver when the objective lens is attached to the objective lens attaching unit.
7. The microscope according to claim 6 , wherein the inner focus unit includes one of more rotation mechanisms that change at least one of a position and a direction of the objective optical axis of the objective optical system attached to the tip thereof.
8. The microscope according to claim 7 , wherein the microscope is a gate-type microscope.
9. The microscope according to claim 6 , wherein:
the variable-focus optical system is an electrically tunable lens (ETL), and
the ETL is disposed so as to maintain an optical axis direction of the ETL in a vertical direction.
10. The microscope according to claim 6 , wherein the microscope is a two-photon laser scanning microscope, a laser scanning microscope, or a disk scanning confocal microscope.
11. A microscope comprising:
an objective optical system that condenses light from a sample;
a relay optical system that relays the light condensed by the objective optical system;
a photodetector that detects the light coming from the objective optical system and relayed by the relay optical system;
a variable-focus optical system disposed at a position between the photodetector and the objective optical system, the position being optically conjugate to a pupil of the objective optical system, and the variable-focus optical system being capable of changing a focal position of the objective optical system in a direction along an objective optical axis;
a tube lens that condenses the light from the sample; and
a pupil projection lens that converts the light condensed by the tube lens into substantially parallel light,
wherein:
the relay optical system includes a pair of relay lenses disposed between the tube lens and the pupil projection lens, and
the variable-focus optical system is disposed between the relay lenses.
12. The microscope according to claim 11 , wherein:
the variable-focus optical system is an electrically tunable lens (ETL), and
the ETL is disposed so as to maintain an optical axis direction of the ETL in a vertical direction.
13. The microscope according to claim 11 , wherein the microscope is a two-photon laser scanning microscope, a laser scanning microscope, or a disk scanning confocal microscope.
14. A microscope comprising:
an objective optical system that condenses light from a sample;
a relay optical system that relays the light condensed by the objective optical system;
a photodetector that detects the light coming from the objective optical system and relayed by the relay optical system;
a variable-focus optical system disposed at a position between the photodetector and the objective optical system, the position being optically conjugate to a pupil of the objective optical system, and the variable-focus optical system being capable of changing a focal position of the objective optical system in a direction along an objective optical axis;
a microscope body; and
an inner focus unit detachably attached to the microscope body, the inner focus unit having the objective optical system attached to a tip thereof,
wherein:
the inner focus unit includes the relay optical system and the variable-focus optical system, and
the inner focus unit includes one or more rotation mechanisms that change at least one of a position and a direction of the objective optical axis of the objective optical system attached to the tip thereof.
15. The microscope according to claim 14 , wherein the microscope is a gate-type microscope.
16. The microscope according to claim 14 , wherein:
the variable-focus optical system is an electrically tunable lens (ETL), and
the ETL is disposed so as to maintain an optical axis direction of the ETL in a vertical direction.
17. The microscope according to claim 14 , wherein the microscope is a two-photon laser scanning microscope, a laser scanning microscope, or a disk scanning confocal microscope.