Methods and devices for using mucolytic agents including N-acetyl cysteine (NAC)
Devices and methods incorporate mucolytic agents into a point-of-care testing device. The sample is loaded, and then the sample travels until it encounters one or more lysis agents and/or mucolytic agents. The mucolytic agent is preferably pre-loaded onto the collection device. In a preferred embodiment, the mucolytic agent is localized between the sample application zone and the conjugate zone. In embodiments with a sample compressor, one or more mucolytic agents may be pre-loaded and dried on the sample compressor, the sample collector, in various locations on the test strip, or in the running buffer.
1. A method for detecting an extracellular target and an intracellular target in a sample, on a sample analysis device that includes a flow of an elution medium from an elution medium application zone to a detection zone, comprising the steps of:
a) transferring a blood sample that includes intact cells onto a sample application zone on the sample analysis device wherein the extracellular target is typically found in extracellular fluid in vivo and the intracellular target is typically found within the intact cells in vivo;
b) applying an elution medium that includes a lysis agent capable of lysing the cells to the elution medium application zone, such that the elution medium including the at least one lysis agent flows through the sample application zone toward the detection zone lysing the intact cells and thereby releasing the intracellular target that is present within the intact cells into the elution medium prior to reaching the detection zone;
c) detecting the presence of the intracellular target at the detection zone on the sample analysis device; and
d) detecting the presence of the extracellular target at the detection zone on the sample analysis device.
2. The method of claim 1 , wherein a conjugate zone is located between the sample application zone and the detection zone.
3. The method of claim 1 , wherein the sample is whole blood.
4. The method of claim 1 , wherein the device is a lateral flow immunoassay device.
5. The method of claim 1 , wherein the device is a chromatography test strip.
6. The method of claim 1 , further comprising a blocking zone comprising a barrier that slows or arrests lysed materials effectively larger than a porosity of the barrier.
7. The device of claim 6 , wherein the barrier is selected from the group consisting of a physical barrier and a biological barrier.