Production of renewable hydrocarbon compositions

1. A method for producing a renewable hydrocarbon composition comprising isobutanol, the method comprising:

a) providing a feedstock slurry comprising protein hydrolysates and fermentable carbohydrate;

b) adding the feedstock slurry to a fermentation medium;

c) contacting the fermentation medium comprising the feedstock slurry with a recombinant yeast host cell capable of producing a renewable hydrocarbon composition from both protein hydrolysates and fermentable carbohydrate; and

d) recovering a renewable hydrocarbon composition from the fermentation medium by distillation;

wherein the recombinant yeast host cell comprises:

i) an isobutanol production pathway comprising enzymes which catalyze the following substrate to product conversions: pyruvate to acetolactate, acetolactate to 2,3-dihydroxyisovalerate, 2,3-dihydroxyisovalerate to α-ketoisovalerate, α-ketoisovalerate to isobutyraldehyde, and isobutyraldehyde to isobutanol;

ii) at least one heterologous enzyme which catalyzes a substrate to product conversion of i); and

iii) at least one genetic modification which increases the activity of LEU4, at least one genetic modification which increases the activity of LEU9, at least one genetic modification which increases the activity of ILV1, or a combination of said genetic modifications.

2. The method of claim 1 , wherein the at least one heterologous enzyme which catalyzes a substrate to product conversion is acetolactate synthase, ketol-acid reductoisomerase, dihydroxyacid dehydratase, ketoisovalerate decarboxylase, or alcohol dehydrogenase.

3. The method of claim 1 , wherein at least two heterologous enzymes catalyze substrate to product conversions of i).

4. The method of claim 1 , wherein at least three heterologous enzymes catalyze substrate to product conversions of i).

5. The method of claim 1 , wherein the genetic modification which increases the activity of LEU4, LEU9, or ILV1 comprises an upregulation of LEU4, LEU9, or ILV1 genes.

6. The method of claim 1 , wherein the recombinant yeast host cell comprises a disruption of an endogenous polynucleotide encoding a polypeptide having pyruvate decarboxylase activity or an endogenous polypeptide having pyruvate decarboxylase activity.

7. The method of claim 6 , wherein the recombinant yeast host cell comprises a modification to reduce glycerol-3-phosphate dehydrogenase activity.

8. The method of claim 1 , wherein the recombinant yeast host cell comprises modifications in polynucleotides encoding URA3 (orotidine-5′-phosphate decarboxylase), HIS3 (imidazoleglycerol-phosphate dehydratase), FRA2 (iron repressor protein), CCC1 (putative vacuolar Fe2+/Mn2+transporter), GPD2 (glycerol-2-phosphate dehydrogenase) or acetolactate reductase or polypeptides having URA3, HIS3, FRA2, CCC1, GPD2, or acetolactate reductase activity, or combinations thereof.

9. The method of claim 1 , wherein the recombinant yeast host cell is Saccharomyces, Schizosaccharomyces, Hansenula, Candida, Kluyveromyces, Yarrowia, Issatchenkia , or Pichia.

10. The method of claim 1 , wherein the renewable hydrocarbon composition comprises isobutanol.

11. The method of claim 1 , further comprising blending an amount of the renewable hydrocarbon composition with gasoline whereby a fuel blend is produced.