Targeted enzymatic degradation of quorum-sensing peptides
Methods and compositions for the treatment of biofilms and/or the inhibition of biofilm formation. In one embodiment, a biofilm is treated and/or biofilm formation is inhibited by a method comprising contacting a biofilm or a surface with a bifunctional ligand comprising a quorum-sensing-peptide-binding region and a protease-binding region, whereby the biofilm is treated and/or biofilm formation on the surface is inhibited.
1. A dressing for treating a wound, comprising:
a distribution manifold comprising collagen; and
a bifunctional ligand disposed on a surface of the distribution manifold and comprising a quorum-sensing-molecule-binding region chemically linked to a protease-binding region.
2. The dressing of claim 1 , wherein the distribution manifold further comprises oxidized regenerated cellulose.
3. The dressing of claim 1 , further comprising a sealing member adapted to be positioned over the distribution manifold and the wound to create a sealed environment.
4. The dressing of claim 1 , further comprising:
a sealing member adapted to be positioned over the distribution manifold and the wound to create a sealed environment; and
a reduced-pressure source adapted to be in fluid communication with the sealed environment.
5. The dressing of claim 1 , wherein the quorum-sensing-molecule-binding region is adapted to bind at least one of an acylated homoserine lactone (AHL), an autoinducing peptide (AlP), AI-2 (a furanosyl borate diester), a γ-butyrolactone, and a competence stimulating peptide (CSP).
6. The dressing of claim 1 , wherein the distribution manifold further comprises gelatin.
7. The dressing of claim 1 , wherein the protease-binding region is adapted to bind a lactonase.
8. The dressing of claim 1 , further comprising a peptide linker between the quorum-sensing-molecule-binding region and the protease-binding region.
9. A system for treating a tissue site, comprising:
a dressing comprising a bioresorbable material and a bifunctional ligand comprising a quorum-sensing-molecule-binding region chemically linked to a protease-binding region;
a sealing member adapted to be positioned over the dressing and the tissue site to create a sealed environment; and
a reduced-pressure source adapted to be in fluid communication with the sealed environment.
10. The system of claim 9 , wherein the bioresorbable material comprises collagen.
11. The system of claim 9 , wherein the bioresorbable material comprises chitosan.
12. The system of claim 9 , wherein the bioresorbable material comprises collagen and oxidized regenerated cellulose.
13. The system of claim 9 , wherein the bioresorbable material comprises at least one of polylactic acid and polyglycolic acid.
14. The system of claim 9 , wherein the bifunctional ligand further comprises a peptide linker between the quorum-sensing-molecule-binding region and the protease-binding region.
15. The system of claim 9 , wherein the dressing further comprises an antibiotic compound.
16. A dressing for treating a wound, comprising:
a carrier material adapted to be positioned on the wound; and
a composition adapted to be applied to the carrier material and comprising:
a bifunctional ligand comprising a quorum-sensing-molecule-binding region chemically linked to a protease-binding region, and
a therapeutic agent for treating the wound.
17. The dressing of claim 16 , wherein the therapeutic agent comprises an antibiotic.
18. The dressing of claim 16 , wherein the therapeutic agent comprises a substance for promoting cell growth.
19. The dressing of claim 16 , wherein the carrier material comprises a polysaccharide.
20. The dressing of claim 16 , wherein the carrier material comprises collagen.