IP Library Granted Patent US 10,712,244
Granted Patent B2
US 10,712,244 · App. 15/814,719 · Granted Jul 14, 2020

Specimen staining apparatus and specimen staining method

Inventors: Yoshihiro Kawano (Tokyo, JP); Kei Tsuyuki (Tokyo, JP); Asuka Yamamoto (Tokyo, JP)
Assignee: OLYMPUS CORPORATION
G01N1/312B01L3/5027B01L2300/0822B01L2400/0478G01N1/44G01N2001/317
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Quick Facts
Patent No.
US 10,712,244
App. No.
15/814,719
Granted
Jul 14, 2020
Kind
B2
Abstract

Provided is a specimen staining apparatus that includes a gap-forming member arranged to form a gap having a thickness that induces capillary action, the gap being formed above a mounting surface of a slide glass having a specimen mounted thereon; and a chemical solution supply unit that supplies a chemical solution for staining the specimen to the gap between the gap-forming member and the mounting surface so that the chemical solution spreads within the gap due to capillary action and is evenly supplied to the specimen placed on the mounting surface.

Claims (21)

1. A specimen staining apparatus comprising:

a gap-forming member arranged to form a gap that has a pre-set, fixed size and that induces capillary action, the gap being formed above a mounting surface of a slide glass having a specimen mounted thereon,

wherein the gap-forming member includes:

a plurality of tanks that contain different types of chemical solutions for staining the specimen; and

a plurality of pressurizing portions, each of the pressurizing portions pressurizing a respective one of the chemical solutions in a respective one of the plurality of tanks,

wherein each of the chemical solutions is supplied to the gap formed between the gap-forming member and the mounting surface, and

wherein each of the plurality of tanks includes a discharge port through which the chemical solution inside the tank is discharged into the gap when the chemical solution is pressurized.

2. The specimen staining apparatus according to claim 1 , further comprising:

a washing fluid supplying portion that supplies a washing fluid to the gap formed between the gap-forming member and the mounting surface; and

a washing fluid discharging portion that discharges, from the gap, the washing fluid supplied by the washing fluid supplying portion.

3. The specimen staining apparatus according to claim 2 , further comprising a heating portion that heats the specimen placed on the slide glass.

4. The specimen staining apparatus according to claim 1 , further comprising a pressure-reducing portion that reduces a pressure in the gap.

5. The specimen staining apparatus according to claim 1 , wherein the discharge port includes a valve that opens by application of pressure.

6. A specimen staining method comprising placing a gap-forming member so that a gap that has a pre-set, fixed size and that induces capillary action is formed between the gap-forming member and a mounting surface of a slide glass having a specimen mounted thereon, and supplying, from a plurality of tanks that contain different types of chemical solutions, the chemical solutions for staining the specimen to the gap formed between the gap-forming member and the mounting surface.

7. A specimen staining apparatus comprising:

container including an opening in which a slide glass having a specimen mounted thereon is placed;

a cover arranged on the opening of the container; and

a plurality of tanks that contain different types of chemical solutions for staining the specimen,

wherein the cover forms a gap having a pre-set, fixed size above an upper surface of the slide glass placed in the opening so as to induce capillary action within the gap, and

wherein each of the chemical solutions is discharged into the gap via a respective discharge port formed in the cover when the chemical solution is pressurized.

8. The specimen staining apparatus according to claim 7 , wherein each of the plurality of tanks comprises a bag-shaped chemical container.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 25, 2023
From: OLYMPUS CORPORATION
To: EVIDENT CORPORATION
Reel/Frame 062492/0267 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 16, 2017
From: KAWANO, YOSHIHIRO; TSUYUKI, KEI; YAMAMOTO, ASUKA
To: OLYMPUS CORPORATION
Reel/Frame 044150/0883 →