IP Library › Granted Patent US 10,294,464
Granted Patent B2
US 10,294,464 · App. 15/824,413 · Granted May 21, 2019

High fidelity restriction endonucleases

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Quick Facts
Patent No.
US 10,294,464
App. No.
15/824,413
Granted
May 21, 2019
Kind
B2
Abstract

Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer.

Claims (2)

1. A composition comprising a variant NsiI restriction endonuclease having reduced star activity, wherein the variant NsiI restriction endonuclease comprises an amino acid sequence that differs from the amino acid sequence of the parent NsiI restriction endonuclease by an amino acid substitution at a position corresponding to position 376 in SEQ ID NO:10.

2. The composition of claim 1 , wherein the substitution is F376A.

Assignments (2)
NOTICE OF GRANT OF SECURITY INTEREST IN PATENTS Recorded Sep 27, 2023
From: NEW ENGLAND BIOLABS, INC.
To: BANK OF AMERICA, N.A., AS ADMINISTRATIVE AGENT
Reel/Frame 065044/0729 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 7, 2017
From: ZHU, ZHENYU; QUIMBY, AINE; GUAN, SHENGXI; SUN, DAPENG; HUANG, YISHU; LAI, XUHUI; CHAN, SIU-HONG; LI, XIANGHUI; XU, SHUANG-YONG; ZHANG, CHUNHUA
To: NEW ENGLAND BIOLABS, INC.
Reel/Frame 044326/0253 →