CELL COMPOSITION DEPLETED FROM TCRab and CD45RA POSITIVE CELLS
The invention is directed to a method for the preparation of a cell population from a sample originating from bone marrow or blood, comprising the steps a) dividing the sample into a first fraction containing 50 to 99% of the cells of the sample and a second fraction containing 50 to 1% of the cells of the sample, b) labeling the cells of the first fraction with a first marker against TCR alpha/beta, c) labeling the cells of the second fraction with a second marker against CD45RA, d) removing the labeled cells from the first and second fraction and combining the remaining cells to a cell population. Furthermore, the invention is directed to a cell composition and the use of the cell composition
1 . A method for the preparation of a cell population from a sample originating from bone marrow or blood, comprising the steps a) dividing the sample into a first fraction containing 50 to 99% of the cells of the sample and a second fraction containing 50 to 1% of the cells of the sample, b) labeling the cells of the first fraction with a first marker against TCR alpha/beta, c) labeling the cells of the second fraction with a second marker against CD45RA, d) removing the labeled cells from the first and second fraction and combining the remaining cells to a cell population.
2 . The method according to claim 1 , wherein the first marker comprises an antibody or antigen-binding fragment against TCR alpha/beta and a detection moiety.
3 . The method according to claim 1 , wherein the second marker comprises an antibody or antigen-binding fragment against CD45RA and a detection moiety.
4 . The method according to claim 1 , characterized in labeling cells of the first fraction and/or the second fraction and/or the cell population with a third marker against CD19 and removing the labeled cells.
5 . The method according to claim 4 , wherein the third marker comprises an antibody or antigen-binding fragment against CD19 and a detection moiety.
6 . The method of the claim 1 , wherein the detection moiety of the first and/or second and/or third marker is a fluorescence dye, a magnetic particle or a radioactive label.
7 . The method of the claim 1 , wherein in step d), first and second fraction are combined and the labeled cells are removed from the combined fraction.
8 . The method of claim 1 , wherein in step d), labeled cells are removed from the first and second fraction separately and the remaining cells of each fraction are combined.
9 . A pharmaceutical composition, comprising a cell population obtainable from bone marrow, whole blood or processed blood, wherein the cell population is depleted of TCR alpha/beta positive cells and CD45RA positive cells by 0.1 to 7.0 log.
10 . The pharmaceutical composition according to claim 9 , wherein the cell population is obtained with the method according to claim 1 .
11 . A use of the pharmaceutical composition according to claim 9 for the reconstitution of the hematopoietic system of a human after a stem cell or bone marrow transplantation.
12 . A use of the pharmaceutical composition according to claim 9 for the prevention and treatment of infection, mixed chimerism, cancer recurrence, or immune disorders.