GASTROINTESTINAL TRACT DETECTION METHODS, DEVICES AND SYSTEMS
The present disclosure relates to gastrointestinal (GI) tract detection methods, devices and systems.
1 . A composition, comprising:
a dye; and
a reagent capable of selectively lysing eukaryotic cells.
2 .- 130 . (canceled)
131 . The composition of claim 1 , wherein the dye is capable of binding to or reacting with a target component of a viable cell.
132 . The composition of claim 131 , wherein the dye exhibits fluorescence that is measurably altered when the dye is bound to or reacted with the target component of the viable cell.
133 . The composition of claim 11 , wherein the dye exhibits fluorescence when bound to a nucleic acid.
134 . The composition of claim 1 , wherein the dye is a lipophilic dye that exhibits fluorescence when metabolized by a cell.
135 . The composition of claim 1 , wherein the dye exhibits fluorescence when reduced by a cell or a cell component.
136 . The composition of claim 1 , wherein the dye comprises a member selected from the group consisting of resazurin, C 12 -resazurin, 7-hydroxy-9H-(1,3 dichloro-9,9-dimethylacridin-2-ol) N-oxide, 6-chloro-9-nitro-5-oxo-5H-benzo[a]phenoxazine, and a tetrazolium salt.
137 . The composition of claim 1 , wherein the dye exhibits fluorescence when oxidized by a cell or a cell component.
138 . The composition of claim 1 , wherein the dye exhibits fluorescence when de-acetylated and/or oxidized by a cell or a cell component.
139 . The composition of claim 1 , wherein the dye exhibits fluorescence when reacted with a peptidase.
140 . The composition of claim 1 , wherein the dye comprises a chemiluminescent dye that exhibits chemiluminescence when metabolized by a viable cell.
141 . The composition of claim 1 , wherein the dye comprises luminol.
142 . The composition of claim 1 , wherein the reagent comprises a detergent.
143 . The composition of claim 1 , wherein the reagent comprises a non-ionic detergent.
144 . The composition of claim 1 , further comprising a second reagent capable of selectively lysing eukaryotic cells.
145 . The composition of claim 1 , further comprising an electrolyte.
146 . The composition of claim 1 , further comprising an electrolyte.
147 . An article, comprising:
a member comprising an absorptive material; and
a composition comprising a dye and a reagent capable of selectively lysing eukaryotic cells,
wherein the composition is at least partially absorbed in the absorptive material.
148 . The article of claim 147 , wherein the absorptive material comprises a sponge.
149 . A device, comprising:
a member comprising an absorptive material; and
a composition comprising a dye and a reagent capable of selectively lysing eukaryotic cells,
wherein the composition is at least partially absorbed in the absorptive material, and the device is an ingestible device.
150 . A method, comprising:
disposing a sample in a device according to claim 149 , thereby producing a product; and
after disposing the sample is in the device, measuring a parameter selected from total fluorescence of the sample, and a rate of change of fluorescence as a function of time of the product in the sample.