Cell suspension preparation technique and device

1. A kit comprising:

a heater;

a base portion configured to receive a container to be heated by the heater;

a first member located within the base portion, wherein the first member comprises a compartment;

a second member comprising a lid structure and one or more sidewalls extending from the lid structure, wherein the second member has a first configuration in which the lid structure covers at least a portion of the first member, and a second inverted configuration in which the one or more sidewalls extend from the lid structure to form a reservoir area, wherein the reservoir area is of sufficient size to permit manipulation of a skin tissue sample; and

a set of tools for cell harvesting, comprising an enzyme for dissociating cellular stratum in the skin tissue sample from a patient and a nutrient solution for a cell suspension, wherein the nutrient solution is configured to maintain cellular viability.

2. The kit of claim 1 , wherein the set of tools further comprises a sterile solution for suspension of the enzyme to provide an enzyme solution.

3. The kit of claim 1 , wherein the first member further comprises the heater, and the heater comprises one or more heating pads positioned on top of the first member.

4. The kit of claim 1 , wherein the heater is housed within a recess in the first member.

5. The kit of claim 4 , wherein the recess further comprises the container located therein into which the skin tissue sample is placed for exposure to an enzyme solution.

6. The kit of claim 1 , wherein the first member comprises a filter recess configured to receive and hold a tube into which a plume of cells harvested from the skin tissue sample is filtered.

7. The kit of claim 1 , wherein the first member comprises a fluid containment well configured to hold the nutrient solution.

8. The kit of claim 7 , wherein the fluid containment well is of sufficient size to submerge the skin tissue sample in the nutrient solution.

9. The kit of claim 1 , wherein the first member provides a seat upon which the second member is placed during manipulation of the skin tissue sample.

10. The kit of claim 1 , wherein the compartment is a storage compartment in which the set of tools may be stored.

11. The kit of claim 10 , wherein the lid structure covers the compartment in the first configuration.

12. The kit of claim 1 , wherein the set of tools further comprises a vessel of the enzyme, a sterile solution for suspension of the enzyme, the nutrient solution, a scalpel, forceps, a syringe, a medicine dropper, a cell filter, and/or wound dressings.

13. The kit of claim 1 , wherein the set of tools comprises a tool for applying the cell suspension to a graft site of a patient.

14. The kit of claim 13 , wherein the tool for applying the cell suspension is a spray nozzle, a pipette, an eye-dropper, or a syringe and needle.

15. The kit of claim 1 , wherein the heater is housed in the base portion.

16. The kit of claim 1 , wherein the base portion comprises a containment field sized to fit the container.

17. The kit of claim 16 , wherein the heater comprises a heating collar located between the containment field and the container.

18. The kit of claim 17 , wherein the base portion further comprises a circuit board in electrical communication with the heating collar.

19. The kit of claim 1 , wherein the heater is configured to heat an enzyme solution to a working temperature, wherein upon incubating the skin tissue sample in the heated enzyme solution, the cellular stratum of the skin tissue sample is dissociated.

20. The kit of claim 19 , wherein the working temperature is between 30° C. and 37° C.

21. The kit of claim 20 , wherein the working temperature is between 33° C. and 37° C.

22. The kit of claim 19 , wherein the heater is configured to heat the enzyme solution to 37° C. within 2 minutes.

23. The kit of claim 19 , wherein the heater is configured to maintain the working temperature for at least 45 minutes.