Method and associated device for rapid detection of target biomolecules with enhanced sensitivity
A rapid detection method of a target biomolecule comprising an antigenic moiety is provided. The method includes providing a source biological sample comprising the target biomolecule; contacting the source biological sample to an ion-exchange medium; eluting the captured-target biomolecule from the ion-exchange medium as an eluate, and loading the eluate to a rapid diagnostic testing device comprising an antibody. The eluate comprises a concentrated form of the biomolecule in a solution having a salt concentration greater than 150 mM. A concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the biomolecule in the source biological sample. The target biomolecule binds to the antibody under the salt concentration of greater than 150 mM. A device for rapid detection of target biomolecule is also provided.
1. A rapid detection method of a target biomolecule, comprising:
contacting a source biological sample comprising the target biomolecule to an ion-exchange medium comprising one or more ligands to capture the target biomolecule and form a captured-target biomolecule, the target biomolecule comprising an antigenic moiety;
eluting, using an elution buffer having a buffer salt concentration greater than 150 mM, the captured-target biomolecule from the ion-exchange medium as an eluate comprising a concentrated form of the target biomolecule in a solution, wherein a concentration of the target biomolecule in the eluate is in a range from about 2× to 25× compared to a concentration of the target biomolecule in the source biological sample, and wherein the solution has a salt concentration greater than 150 mM; and
loading the eluate comprising the concentrated form of the target biomolecule in the solution to a rapid diagnostic testing device comprising an antibody, wherein the target biomolecule binds to the antibody under the salt concentration of greater than 150 mM.
2. The method of claim 1 , wherein the target biomolecule is a glycolipid.
3. The method of claim 1 , wherein the target biomolecule is a tuberculosis-lipoarabinomannan (TB-LAM).
4. The method of claim 1 , wherein the target biomolecule binds to the antibody under the salt concentration in a range from about 0.5M to about 2M.
5. The method of claim 1 , wherein the target biomolecule binds to the antibody under the salt concentration of about 1.3M.
6. The method of claim 1 , wherein the elution of the captured-target biomolecule from the ion-exchange medium is effected under a salt concentration in a range from about 0.5M to about 2M.
7. The method of claim 1 , wherein the eluate comprising the concentrated form of the target biomolecule is loaded without any dilution to the rapid diagnostic testing device comprising an antibody.
8. The method of claim 1 , further comprising diluting the source biological sample comprising the target biomolecule by at least 2× to form a diluted biological sample.
9. The method of claim 1 , further comprising diluting the source biological sample comprising the target biomolecule by 4× to form a diluted biological sample.
10. The method of claim 1 , wherein the rapid diagnostic testing device comprises a lateral flow assay (LFA) device, an enzyme linked immuno-sorbent assay (ELISA) device, or a combination thereof.
11. The method of claim 10 , wherein the rapid diagnostic testing device comprises a lateral flow assay (LFA) device.
12. The method of claim 1 , wherein the source biological sample comprises urine, blood, feces, sweat, saliva, mucous, milk, semen, serum, plasma, sputum, tears, tissue, or combinations thereof.
13. The method of claim 12 , wherein the source biological sample is urine.
14. The method of claim 1 , wherein the ion-exchange medium comprises an ion exchange material, an ion-exchange membrane, or an ion-exchange matrix.
15. The method of claim 1 , wherein the one or more ligands comprise anionic ligands.
16. The method of claim 15 , wherein the anionic ligands are quaternary ammonium ion, or dimethyl aminoethyl (DMAE) groups.