IP Library Granted Patent US 12,302,902
Granted Patent B2
US 12,302,902 · App. 15/861,159 · Granted May 20, 2025

Antimicrobial compositions, including antimicrobial hydrogels, effective against mature biofilms

Inventor: Robert DiLuccio (Haymarket, VA)
Assignee: CorMedix Inc.
A01N43/88A01N37/02C07D285/18C08B37/0072C08J3/075C09D5/14C09D5/16C09D105/08A61L2400/18
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Quick Facts
Patent No.
US 12,302,902
App. No.
15/861,159
Granted
May 20, 2025
Kind
B2
Abstract

A method for the prevention or elimination of biofilm microorganisms on at least one surface of a medical device, the method comprising the steps of: (a) providing a biofilm-active composition having at least one biofilm-active agent; and (b) delivering the biofilm-active composition to the medical device in an amount sufficient to prevent or eliminate the biofilm microorganisms on at least one surface of the medical device.

Claims (7)

1. A method for killing Pseudomonas aeruginosa on at least one surface of a catheter, the method comprising the steps of: (a) providing a bio-film active composition comprising: taurolidine, wherein the concentration of the taurolidine, is 0.5% by weight, 1.0% by weight, 1.5% by weight, or 3.0% by weight of the total weight of the bio-film active composition;

a biofilm-penetrating agent comprising myristic acid, wherein the concentration of the myristic acid is about 1.5% by weight of the total weight of the bio-film active composition; and a base material, wherein the base material comprises a hydrogel comprising hyaluronic acid having a molecular weight of 750 kDa to 1.0 MDa; and

(b) delivering the bio-film active composition to the at least one surface of a catheter in a sufficient amount and for a sufficient period of time to kill Pseudomonas aeruginosa on at least one surface of the catheter.

2. A method according to claim 1 wherein the biofilm-active composition is formed by mixing taurolidine, the myristic acid and the base material.

3. A method according to claim 1 wherein the biofilm-active composition is delivered to the at least one surface of the catheter by contacting the at least one surface of the catheter for a period of time sufficient to kill Pseudomonas aeruginosa on the at least one surface of the catheter.

4. A method according to claim 1 wherein the biofilm-active composition is delivered to the at least one surface of the catheter by integrating the biofilm-active composition within the catheter.

5. A method according to claim 1 wherein the hyaluronic acid is crosslinked hyaluronic acid.

Assignments (3)
RELEASE OF SECURITY INTEREST Recorded Aug 20, 2020
From: MANCHESTER SECURITIES CORP.
To: CORMEDIX INC.
Reel/Frame 053560/0152 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 29, 2020
From: DILUCCIO, ROBERT
To: CORMEDIX INC.
Reel/Frame 051661/0102 →
SECURITY AGREEMENT Recorded Jan 2, 2019
From: CORMEDIX INC.
To: MANCHESTER SECURITIES CORP.
Reel/Frame 048000/0571 →
Continuity (2)
Provisional Application 62442775 · Jan 5, 2017
Related Publication 20180184656A1 · Jul 5, 2018
References Cited (47)
US 6475434B1 · Darouiche · 2002 [cited by applicant]
US 7314857B2 · Madhyastha · 2008 [cited by applicant]
US 8529935B2 · Giammona · 2013 [cited by examiner]
US 20040156908A1 · Polaschegg · 2004 [cited by applicant]
US 20050049181A1 · Madhyastha · 2005 [cited by applicant]
US 20110311647A1 · Gawande et al. · 2011 [cited by applicant]
US 20130084319A1 · Priewe et al. · 2013 [cited by applicant]
US 20130085469A1 · Polaschegg · 2013 [cited by applicant]
US 20130129800A1 · Giammona et al. · 2013 [cited by applicant]
US 20130302390A1 · Davies · 2013 [cited by applicant]
US 20150182667A1 · Guelcher et al. · 2015 [cited by applicant]
US 20170100407A1 · Reidenberg · 2017 [cited by examiner]
US 20190046488A1 · Rosenblatt · 2019 [cited by examiner]
EP 1442753 · 2004 [cited by applicant]
KR 20150110843A · 2015 [cited by examiner]
WO WO0033895 · 2000 [cited by applicant]
WO WO2005115357 · 2005 [cited by applicant]
WO WO2008043175 · 2008 [cited by applicant]
WO WO2008143889 · 2008 [cited by applicant]
WO WO2012014180 · 2012 [cited by applicant]
WO WO2013009998 · 2013 [cited by applicant]
WO WO2013049149 · 2013 [cited by applicant]
WO WO2015168677 · 2015 [cited by applicant]
Anwar, H. et al., Dynamic interactions of biofilms of mucoid Pseudomonas aeruginosa with tobramycin and piperacillin, Antimicrob. Agents Chemother., vol. 36, 1992, pp. 1208-1214. [cited by applicant]
Carrel, M. et al., Biofilm imaging in porous media by laboratory X-Ray tomography: Combining a non-destructive contrast agent with propagation-based phass-contrast imaging tool, PLoS ONE, vol. 12, No. 7, e0180374, 2017,… [cited by applicant]
Cirioni et al., Daptomycin and Rifampin Alone and in Combination Prevent Vascular Graft Biofilm Formation and Emergence of Antibiotic Resistance in a Subcutaneous Rate Pouch Model of Staphylocoocal Infection, European J… [cited by applicant]
Darouiche, R.O. et al., A comparison of two antimicrobial-impregnanted central venous catheters, N. Engl. J. Med., vol. 340, No. 1, 1999, pp. 1-8. [cited by applicant]
Hausner, M. et al., High Rates of Conjugation in Bacterial Biofilms as Determined by Quantitative In Situ Analysis, Applied and Environmental Microbiolgy, vol. 65, No. 8, 1999, pp. 3710-3713. [cited by applicant]
Maki, D.G., In Vitro Studies of a Novel Antimicrobial Luer-Activated Needleless Connector for Prevention of Catheter-Related Bloodstream Infection, Clinical Infection Diseases, vol. 50, Iss. 12, 2010, pp. 1580-1587. [cited by applicant]
Roberts. A.P. et al., Characterization of the Ends of Target Site of a Novel Tetracycline Resistance-Encoding Conjugative Transposon from Enterococcus faecium 644.1H1, J. of Bacteriology, vol. 168, No. 12. 2006, pp. 435… [cited by applicant]
Stickler, D.J. et al., The Structure of Urinary Catheter Encrusting Bacterial Biofilms, Cells and Materials, vol. 3, Iss. 3, 1993, pp. 315-320. [cited by applicant]
Brisset, L. et al., In vivo and in vitro analysis of the ability of urinary catheter to microbial colonization, Pathol Biol (Paris), vol. 44, No. 5, 1996, pp. 397-404. [cited by applicant]
Costerton, J.W. et al., Bacterial Biofilms: A Common Cause of Persistent Infections, Science, vol. 284, 1999, pp. 1318-1322. [cited by applicant]
Elliot, T.S.J., Novel approach to investigate a source of microbial contamination of central venous catheters, Eur. J. Clin. Microbiol. Infect. Dis., vol. 16, No. 3, 1997. pp. 210-213. [cited by applicant]
Flowers, R.H. et al., Efficacy of an attachable subcutaneous cuff for the prevention of intravascular catheter-related infection, JAMA, vol. 261, No. 6. 1989, pp. 878-883. [cited by applicant]
Kamal, G.D. et al., Reduced intravascular catheter infection by antibiotic bonding, A prospective, randomized, controlled trial, JAMA, vol. 265, 1991, pp. 2364-2368. [cited by applicant]
Morris, N.S. et al., Encrustation of indwelling urethral catheters by Proteus mirabilis biofilms growing in human urine, J. Hosp. Infect., vol. 39, Iss. 3, 1998, pp. 227-234. [cited by applicant]
Raad, I., Intravascular-catheter-related infections, Lancet., vol. 351, No. 9106, 1998, pp. 893-898. [cited by applicant]
Raad, I. et al., Quantitative tip culture methods and the diagnosis of central venous catheter-related infections, Diagn. Microbial. Infect. Dis., vol. 15, Iss. 1, 1992, pp. 13-20. [cited by applicant]
Tunney, M.M. et al., Biofilm and biofilm-related encrustation of urinary tract devices, Methods Enzymol., vol. 310, 1999, pp. 558-566. [cited by applicant]
Drago et al., “Antiadhesive and antibiofilm activity of hyaluronic acid against bacteria responsible for respiratory tract infections”, APMIS, vol. 122, No. 10, 2014, pp. 1013-1019. [cited by applicant]
Perez-Giraldo et al. “Influence of N-acetylcysteine on the formation of biofilm by [cited by applicant]
Koo et al., “Targeting microbial biofilms: current and prospective therapeutic strategies”, Nat Rev Microbiol, vol. 15, No. 12, 2017, pp. 740-755. [cited by applicant]
Rodriguez-Beltran et al., “N-Acetylcysteine Selectively Antagonizes the Activity of Imipenem in Pseudomonas aeruginosa by an OprD-Mediated Mechanism”, Antimicrobial Agents and Chemotherapy, vol. 59, No. 6, 2015, pp. 324… [cited by applicant]
Liaw S. et al., Modulation of Swarming and Virulence by Fatty Acids through the RsbA Protein in Proteus mirabilis, Infection and Immunity, Dec. 2004, vol. 72, No. 12, pp. 6836-6845. [cited by applicant]
Inoue T. et al., Inhibition of swarming motility of Pseudomonas aeruginosa by branched-chain fatty acids, FEMS Microbiology Letters, Mar. 2008, vol. 281, No. 1, pp. 81-86. [cited by applicant]
Cassandro E. et al., Hyaluronan in the Treatment of Chronic Rhinosinusitis with Nasal Polyposis, Indian Journal of Otolaryngology and Head & Neck Surgery, Sep. 2015, vol. 67, No. 3, pp. 299-307. [cited by applicant]