Microscope apparatus
A microscope apparatus 10 includes a detection optical system 12 that captures light from a sample S and an illumination optical system 11 that radiates an illumination light onto the sample S. The illumination optical system 11 includes a cylindrical lens 5 that has a power in a first-axis direction and does not have a power in a second-axis direction that is perpendicular to the first-axis direction, a cylindrical lens 6 that has a power in the second-axis direction and does not have a power in the first-axis direction, and a scanner 4 that scans the illumination light in a width direction. The illumination optical system 11 is configured such that the first-axis direction is the width direction described above, and the cylindrical lenses 5 and 6 are arranged posterior to the scanner 4.
1. A microscope apparatus comprising:
a detection optical system that captures light from a sample; and
an illumination optical system that has an optical axis oriented in a direction different from an optical axis of the detection optical system and that radiates an illumination light onto the sample,
wherein:
the illumination optical system includes:
a first optical-axis-asymmetric optical system that has a power in a first-axis direction and does not have a power in a second-axis direction that is perpendicular to the first-axis direction,
a second optical-axis-asymmetric optical system that has a power in the second-axis direction and does not have a power in the first-axis direction, and
a scanning unit that scans the illumination light in a width direction that is perpendicular to the optical axis of the detection optical system and the optical axis of the illumination optical system;
the illumination optical system is configured such that the first-axis direction is the width direction;
the first optical-axis-asymmetric optical system and the second optical-axis-asymmetric optical system are arranged between the sample and the scanning unit; and
the illumination optical system concentrates the illumination light onto a substantially same position in the optical axis of the illumination optical system by each of the first optical-axis-asymmetric optical system and the second optical-axis-asymmetric optical system so as to form an optical spot within a field of view of the detection optical system, and scans the optical spot so as to form a sheet light.
2. The microscope apparatus according to claim 1 , wherein the scanning unit comprises a scanner that is arranged in one of a front focal plane of the first optical-axis-asymmetric optical system and a plane conjugate to the front focal plane.
3. The microscope apparatus according to claim 1 , wherein the illumination optical system includes an optical system that converts a beam that enters the scanning unit into a collimated beam.
4. The microscope apparatus according to claim 1 , wherein an emission-side light concentration numerical aperture of the illumination optical system in the first-axis direction is not less than 0.04.
5. The microscope apparatus according to claim 1 , wherein one of the first optical-axis-asymmetric optical system and the second optical-axis-asymmetric optical system includes an aspherical lens.
6. The microscope apparatus according to claim 1 , wherein the optical axis of the detection optical system and the optical axis of the illumination optical system are perpendicular to each other.
7. The microscope apparatus according to claim 1 , further comprising a first numerical-aperture-changing unit that changes an emission-side light concentration numerical aperture of the illumination optical system in the first-axis direction.
8. The microscope apparatus according to claim 7 , wherein:
the illumination optical system includes a third optical-axis-asymmetric optical system that is placed in an optical path of the illumination optical system by switching being performed from the first optical-axis-asymmetric optical system placed in the optical path of the illumination optical system and that provides an emission-side light concentration numerical aperture that is different from an emission-side light concentration numerical aperture of the first optical-axis-asymmetric optical system; and
the first numerical-aperture-changing unit comprises a switching mechanism that performs switching between the first optical-axis-asymmetric optical system and the third optical-axis-asymmetric optical system so as to place one of the first optical-axis-asymmetric optical system and the third optical-axis-asymmetric optical system in the optical path of the illumination optical system.
9. The microscope apparatus according to claim 7 , wherein the first numerical-aperture-changing unit comprises a zoom lens that changes an emission-side light concentration numerical aperture of the first optical-axis-asymmetric optical system.
10. The microscope apparatus according to claim 1 , further comprising a second numerical-aperture-changing unit that changes an emission-side light concentration numerical aperture of the illumination optical system in the second-axis direction.
11. The microscope apparatus according to claim 10 , wherein:
the illumination optical system includes a fourth optical-axis-asymmetric optical system that is placed in an optical path of the illumination optical system by switching being performed from the second optical-axis-asymmetric optical system placed in the optical path of the illumination optical system and that provides an emission-side light concentration numerical aperture that is different from an emission-side light concentration numerical aperture of the second optical-axis-asymmetric optical system; and
the second numerical-aperture-changing unit comprises a switching mechanism that performs switching between the second optical-axis-asymmetric optical system and the fourth optical-axis-asymmetric optical system so as to place one of the second optical-axis-asymmetric optical system and the fourth optical-axis-asymmetric optical system in the optical path of the illumination optical system.
12. The microscope apparatus according to claim 10 , wherein the second numerical-aperture-changing unit comprises a zoom lens that changes an emission-side light concentration numerical aperture of the second optical-axis-asymmetric optical system.
13. The microscope apparatus according to claim 10 , wherein:
the illumination optical system includes a light-blocking member that blocks a portion of the illumination light in the second-axis direction, and
the second numerical-aperture-changing unit comprises a light blocking device that changes a range in which the light-blocking member blocks the portion of the illumination light.
14. An illumination device comprising:
an illumination optical system that includes:
a first optical-axis-asymmetric optical system that has a power in a first-axis direction and does not have a power in a second-axis direction that is perpendicular to the first-axis direction;
a second optical-axis-asymmetric optical system that has a power in the second-axis direction and does not have a power in the first-axis direction; and
a scanning unit that scans an illumination light in a width direction that is perpendicular to an optical axis of the illumination optical system,
wherein:
the illumination optical system is configured such that the first-axis direction is the width direction,
the first optical-axis-asymmetric optical system and the second optical-axis-asymmetric optical system are arranged between the sample and the scanning unit, and
the illumination optical system concentrates the illumination light onto a substantially same position in the optical axis of the illumination optical system by each of the first optical-axis-asymmetric optical system and the second optical-axis-asymmetric optical system so as to form an optical spot within a field of view of a detection optical system, and scans the optical spot so as to form a light sheet.