INSTRUMENT AND CARTRIDGE FOR PERFORMING ASSAYS IN A CLOSED SAMPLE PREPARATION AND REACTION SYSTEM EMPLOYING ELECTROWETTING FLUID MANIPULATION
In one embodiment, a multiplex fluid processing cartridge includes a sample well, a deformable fluid chamber, a mixing well with a mixer disposed therein, a lysis chamber including a lysis mixer, an electrowetting grid for microdroplet manipulation, and electrosensor arrays configured to detect analytes of interest. An instrument for processing the cartridge is configured to receive the cartridge and to selectively apply thermal energy, magnetic force, and electrical connections to one or more discrete locations on the cartridge and is further configured to compress the deformable chamber(s) in a specified sequence.
1 - 20 . (canceled)
21 . A method to detect a target analyte comprising:
a) Obtaining an aliquot of sample on a fluidic processing panel comprising electrowetting electrodes;
b) Amplifying the sample in step a by shuttling the sample across a thermal cycling track by electrowetting manipulation;
c) Combining the amplified sample from step b with an amount of the exonuclease;
d) Combining the sample from step c with a signal probe;
e) Transporting by passive diffusion the sample in step d into an electrosensor array; and
f) detecting a target analyte.
22 . The method of claim 21 , wherein the electrosensor array does not comprise electrowetting electrodes.
23 . The method of claim 21 , wherein the sample is encased in a shell of oil.
24 . The method of claim 23 , wherein the electrosensor array comprises a hydrophilic coating.
25 . The method of claim 21 , wherein the combination in step c and d occurs by oscillating movements of the droplets between electrowetting pads.
26 . The method of claim 21 , wherein amplification occurs via multiplex PCR.
27 . The method of claim 21 , wherein amplification and detection occur in different thermal zones.
28 . The method of claim 24 , wherein detecting and Combining the amplified sample from step b with an amount of the exonuclease occur in different thermal zones.
29 . The method of claim 21 , wherein the thermal zone for amplification is at about 95° C. for denaturation and at about 60° C. for annealing and extension.
30 . The method of claim 21 , wherein amplification occurs via multiplex PCR using two different temperature zones.
31 . The method of claim 21 , wherein more than one aliquot of sample can be processed in each thermal cycling track.
32 . The method of claim 28 , wherein the amplification thermal zones are heated by a PCR heater assembly comprising a resistive heating element attached to a dedicated PCB and a heat spreader.
33 . A method to detect a target analyte comprising:
a) Obtaining a droplet comprising amplified nucleic acid bound to a signal probe on a fluidic processing panel;
b) Transporting by electrowetting manipulation the droplet to the detection staging area;
c) Transporting by passive diffusion the droplet in step a to an electrosensor array; and
d) detecting a target analyte.
34 . The method of claim 33 , wherein the fluidic processing panel comprises connector pad arrays.
35 . The method of claim 33 , wherein the fluidic processing panel is coated with a conducting layer.
36 . The method of claim 33 , wherein the fluidic processing panel is coated with a polyimide film.
37 . The method of claim 33 , wherein the fluidic processing panel is coated with a hydrophobic coating.
38 . The method of claim 37 , wherein the electrosensor array is coated with a hydrophilic coating.
39 . A method to detect a target analyte comprising:
a) Obtaining a droplet comprising amplified nucleic acid bound to a signal probe on a fluidic processing panel;
b) Transporting by electrowetting manipulation the droplet in step a from an electrowetting grid comprising electrodes to an electrosensor array comprising detection electrodes;
c) Loading the droplet by passive diffusion into the electrosensor array; and
d) detecting a target analyte.
40 . The method of claim 39 , wherein electrical connections from both the electrowetting electrode grids and detection electrodes extend through the panel to produce a land grid array.