Nucleic acid molecules that control pathogens
This disclosure concerns nucleic acid molecules and methods of use thereof for control of pathogens through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in pathogens. The disclosure also concerns methods for applying dsRNA through formulations and/or transgenic plants that express nucleic acid molecules useful for the control of pathogens, and the plant cells and plants obtained thereby.
1. A double-stranded nucleic acid comprising a ribonucleic acid (RNA) molecule consisting of at least 19 nucleotides, wherein the double-stranded nucleic acid is specifically hybridizable with a polynucleotide selected from the group consisting of:
(a) a polynucleotide sequence of SEQ ID NO:10 and the complement of SEQ ID NO:10; and,
(b) a polynucleotide sequence of SEQ ID NO:11 and the complement of SEQ ID NO:11.
2. The double-stranded nucleic acid of claim 1 , wherein the polynucleotide sequence inhibits or down regulates the expression of an sdh gene endogenous nucleotide sequence specifically complementary to the polynucleotide sequence.
3. The double-stranded nucleic acid of claim 1 , wherein contacting the polynucleotide sequence with a plant pathogen kills or inhibits the growth, reproduction, and/or feeding of the plant pathogen.
4. The plant pathogen of claim 3 , wherein the plant pathogen is selected from the group consisting of Zymoseptoria tritici; Zymoseptoria citri; Zymoseptoria caryae; Zymoseptoria curcurbitacearum; Zymoseptoria dianthi; Zymoseptoria glycines; Zymoseptoria helianthi; Zymoseptoria ostryae; Puccinia triticina; Puccinia striiformis; Phaeosphaeria nodorum; Rhyncosporium commune; Alternaria solani; Cercospora beticola; Magnaporthe grisea; Venturia inaequalis ; and Phakopsora pachyrhizi.
5. The double-stranded nucleic acid of claim 1 , wherein the polynucleotide sequence is operably linked to a heterologous promoter.
6. The double-stranded nucleic acid of claim 1 , wherein the polynucleotide sequence folds back and hybridizes to the complement thereof to form the double stranded molecule.
7. The double-stranded nucleic acid of claim 6 , wherein the double stranded molecule is from 19 to 30 nucleotides in length.
8. The double-stranded nucleic acid of claim 1 , wherein the double-stranded nucleic acid is incorporated into a plasmid vector.
9. The double-stranded nucleic acid of claim 1 , wherein the double-stranded nucleic acid is provided as an oligonucleotide.
10. A composition comprising the double-stranded nucleic acid of claim 1 .
11. A plant comprising the double-stranded nucleic acid of claim 1 .
12. The plant of claim 11 , wherein the plant is a monocot plant or a dicot plant.
13. The plant of claim 11 , wherein the plant is a wheat plant.
14. The plant of claim 11 , wherein the double-stranded nucleic acid is expressed in the plant as a ribonucleic acid molecule, and the ribonucleic acid molecule inhibits or down regulates the expression of a sdh gene endogenous nucleotide sequence specifically complementary to the polynucleotide sequence.
15. A method of making a transgenic plant cell capable of expressing a double-stranded nucleic acid comprising a ribonucleic acid (RNA) molecule that inhibits an sdh gene in a fungal species, said method comprises the steps of transforming a plant cell with the double-stranded nucleic acid of claim 1 , wherein said double-stranded nucleic acid of claim 1 inhibits the sdh gene in the fungal species.
16. A method of controlling a fungus or oomycete, comprising providing to said fungus or oomycete the double-stranded nucleic acid of claim 1 , or a composition comprising at least the double-stranded nucleic acid of claim 1 .