Methods of purifying cannabinoids using liquid:liquid chromatography

1. A method of purifying one or more cannabinoids from a plant material including a plant, a plant resin or a plant extract, the method consisting essentially of the following steps:

(a) incubating the plant material with a solvent selected from the group consisting of pentane, hexane, heptane, petroleum ethers, cyclohexane, dichloromethane, trichloromethane, tetrahydrofurane, diethyl ether, toluene, benzene, ethanol, methanol, isopropanol, acetone, acetonitrile, ethyl acetate, butane, propane, 1,1,1,2-Tetrafluoroethane (R134a), or, liquid, subcritical or supercritical CO 2 or mixes thereof to form a solvent mixture which extracts the one or more cannabinoids from the plant material; wherein the solvent mixture has an original volume and is reduced to dryness or to about 50% or less of the original volume of the solvent mixture;

(b) for THC-type extracts, adding to the solvent mixture a biphasic solvent system selected from the group consisting of hexane:ethanol:water, pentane:acetonitrile and hexane:acetonitrile, wherein the pentane:acetonitrile system and the hexane:acetonitrile system optionally include ethyl acetate and/or water as a modifier; for CBD-type extracts, adding to the extract a biphasic solvent system of hexane:ethanol:water; and for CBG-type extracts, adding to the extract a biphasic solvent system of hexane:ethanol:water; and

(c) performing liquid:liquid chromatography using a biphasic solvent system of step b), wherein the liquid:liquid chromatography is selected from the group consisting of centrifugation partitioning chromatography (CPC) or counter current chromatography (CCC), thereby purifying the one or more cannabinoids.

2. The method of claim 1 , wherein for the THC-type extracts the biphasic solvent system is hexane:ethanol:water at a ratio of (20:17:3) by volume.

3. The method of claim 1 , wherein for the THC-type extracts the biphasic solvent system is pentane:ethyl acetate:acetonitrile:water at a ratio from (10:0:10:0) to (7:3:7:3) by volume.

4. The method of claim 1 , wherein for the THC-type extracts the biphasic solvent system is hexane:ethyl acetate:acetonitrile:water at a ratio from (10:0:10:0) to (7:3:7:3) by volume.

5. The method of claim 1 , wherein for the CBD-type extracts the biphasic solvent system is hexane:ethanol:water at a ratio of (20:14:6) by volume.

6. The method of claim 1 , wherein for the CBG-type extracts the biphasic solvent system is hexane:ethanol:water at a ratio of at a ratio of (20:12:8) or (20:13:7) by volume.

7. The method of claim 1 , wherein an extract of chemotype I or II Cannabis is used to purify THC, THCA, THCV, THCVA, CBN or CBV and fractionate the CBD-type and CBG-type cannabinoids.

8. The method of claim 1 , wherein an extract of chemotype II or III Cannabis is used to purify CBD, CBDA, CBDVA or CBDV and fractionate the THC-type and CBG-type cannabinoids.

9. The method of claim 1 , wherein an extract of chemotype IV Cannabis is used to purify CBG, CBGA, CBGVA or CBGV and fractionate the CBD-type and THC-type cannabinoids.

10. The method according to claim 1 , wherein the solvent mixture of step (a) is purified prior to step (b).

11. The method according to claim 1 , wherein prior to step (a), the one or more cannabinoids present in the plant material are decarboxylated by heating the plant material.

12. The method of claim 1 , wherein after the solvent mixture is reduced to dryness a dry extract product of the solvent mixture is dissolved in ethanol, chilled at a temperature from −20° C. to 4° C. filtered to remove precipitated material and reduced to dryness before purification by liquid-liquid chromatography.

13. The method of claim 1 , using a new rotor design Quantum CPC or CPC 1000 PRO, wherein the rotor has a rotor volume of 1 liter, a sample injection of 50 mL, a flow rate of a mobile phase (hexane phase) of the biphasic solvent system of 200 mL/min during the run, and a flow rate of a stationary phase (the ethanolic phase) of the biphasic solvent system of 350 mL/min during the extrusion phase of the run.

14. The method of claim 1 , using a new rotor design Quantum CPC or CPC 1000 PRO, wherein the total run time is 12-20 minutes, independent of rotor volume.

15. The method of claim 8 , wherein the CBD, CBDA, CBDVA or CBDV is crystalized with a non-polar solvent after the step of liquid:liquid chromatography.

16. The method of claim 9 , wherein the CBG, CBGA, CBGVA or CBGV is crystalized with a non-polar solvent after the step of liquid:liquid chromatography.

17. The method of claim 1 , wherein the plant material is incubated with a non-polar solvent selected from the group consisting of petroleum ether, pentane, hexane and heptane to form a solvent mixture which extracts the one or more cannabinoids from the plant material to form the solvent mixture.

18. The method of claim 1 , wherein the plant material is first incubated with a solvent selected from the group consisting of pentane, hexane, heptane, petroleum ethers, cyclohexane, dichloromethane, trichloromethane, tetrahydrofurane, diethyl ether, toluene, benzene, ethanol, methanol, isopropanol, acetone, acetonitrile, ethyl acetate, butane, propane, 1,1,1,2-Tetrafluoroethane (R134a), or, liquid, subcritical or supercritical CO 2 or mixes thereof; filtered, decanted or centrifuged; reduced to dryness; and then incubated with a non-polar solvent selected from the group consisting of petroleum ether, pentane, hexane and heptane to form a solvent mixture which extracts the one or more cannabinoids from the plant material to form the solvent mixture.

19. The method according to claim 1 , wherein after step (a) the one or more cannabinoids present in the plant material and extracts are decarboxylated by heating a dry extract that contains the one or more cannabinoids from the plant material.