Compositions and methods for RNA-based antimicrobial drug targeting
A ribonucleic acid (RNA) based modular regulatory element (MRE) for inhibiting translation of a target gene transcript in an organism (e.g. a pathogen) includes a first segment of RNA capable of forming a stem loop structure, a second segment of RNA downstream of the first segment of RNA and having a target sequence capable of binding to the target gene transcript (or polycistron lead gene transcript), and a third segment of RNA downstream of the second segment of RNA and being capable of forming a terminator hairpin downstream of the target sequence. Methods for assaying the role of a putative target gene in an organism include inhibiting expression of various target genes using the RNA-based MRE.
1. A ribonucleic acid (RNA) composition for inhibiting translation of a bacterial target gene transcript, the RNA composition comprising:
a first segment of RNA capable of forming a 5′ stem loop structure, the first segment of RNA comprising a sequence of SEQ ID NO: 1, 2, 9, 10, 11, or 12;
a second segment of RNA downstream of the first segment of RNA, the second segment comprising a targeting sequence capable of binding to a ribosomal binding site (RBS) of the bacterial target gene transcript; and
a third segment of RNA downstream of the second segment of RNA, the third segment capable of forming a 3′ terminator downstream of the targeting sequence, the third segment of RNA comprising a sequence of SEQ ID NO: 3.
2. The RNA composition of claim 1 , wherein the targeting sequence is complementary to the RBS of the bacterial target gene transcript.
3. The RNA composition of claim 1 , wherein the bacterial target gene transcript is part of a polycistron.