FMDV and E2 fusion proteins and uses thereof
The present invention encompasses FMDV vaccines or compositions. The vaccine or composition may be a vaccine or composition containing FMDV antigens. The invention also encompasses recombinant vectors encoding and expressing FMDV antigens, epitopes or immunogens which can be used to protect animals, in particular ovines, bovines, caprines, or swines, against FMDV. The invention further encompasses methods of making or producing antigenic polypeptides or antigens.
1. A method of vaccinating a host susceptible to ovine, bovine, caprine, or swine Foot-and-Mouth Disease Virus (FMDV), or protecting or preventing the host against FMDV infection, or eliciting a protective immune response in a host, comprising at least one administration of a composition or vaccine comprising an FMDV antigen or polypeptide; wherein the FMDV antigen or polypeptide has at least 90% sequence identity to a polypeptide having the sequence as set forth in SEQ ID NO:2, 4, 6, 8, 10, 12 or 14.
2. The method of claim 1 , wherein the FMDV antigen or polypeptide is an FMDV-E2 fusion protein.
3. The method of claim 1 or 2 , wherein the method comprises a prime-boost administration regimen.
4. The method of claim 3 , wherein the prime-boost administration comprises a prime-administration of a composition or vaccine comprising an FMDV antigen or polypeptide, and a boost-administration of a vaccine or composition comprising a recombinant viral vector that contains and expresses the FMDV antigen in vivo, or an inactivated viral vaccine comprising the FMDV antigen, or a DNA plasmid vaccine or composition that contains or expresses the FMDV antigen, or a recombinant FMDV antigen produced in plants or algae.
5. The method of claim 3 , wherein the prime-boost administration comprises a prime-administration of a vaccine or composition comprising a recombinant viral vector that contains and expresses the FMDV antigen in vivo, or an inactivated viral vaccine comprising the FMDV, or a DNA plasmid vaccine or composition that contains or expresses the FMDV antigen, or a recombinant FMDV antigen produced in plants or algae, and a boost-administration of a composition or vaccine comprising an FMDV antigen or polypeptide.
6. The method of claim 3 , wherein the prime-boost administration comprises a prime-administration of a composition or vaccine comprising an FMDV antigen or polypeptide, and a boost-administration of a composition or vaccine comprising an FMDV antigen or polypeptide.
7. The method of claim 1 , wherein the host is ovine, bovine, caprine, or swine.
8. A method of producing an antigen comprising the steps of:
i) linking a polynucleotide encoding the antigen to a polynucleotide encoding an E2 protein; ii) expressing the fusion protein in a host; and iii) isolating the fusion protein from the host; wherein the polynucleotide has at least 70% sequence identity to a polynucleotide having the sequence as set forth in SEQ ID NO:1, 3, 5, 7, 9, 11 or 13.