Sample test method, microfluidic device, and test device
A sample test method, microfluidic device, and test device efficiently and accurately compensates for interference of an interfering substance present in a sample using optical measurement without addition of a separate reagent for detecting the interfering substance. The sample test method includes: measuring an optical characteristic value of a target substance present in a sample; measuring an optical characteristic value of an interfering substance present in the sample; and determining a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance.
1. A sample test method comprising:
measuring an optical characteristic value of a target substance present in a sample;
measuring an optical characteristic value of an interfering substance present in the sample; and
determining a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance,
wherein the determining the concentration of the target substance for which interference of the interfering substance is compensated for comprises:
determining the concentration of the target substance based on the optical characteristic value of the target substance, and
determining the concentration of the interfering substance based on the optical characteristic value of the interfering substance, and
compensating the concentration of the target substance using the concentration of the interfering substance.
2. The sample test method of claim 1 , wherein the compensating the concentration of the target substance comprises:
applying a fluctuation coefficient to the concentration of the interfering substance to obtain an application result, and then subtracting or adding the application result from or to the concentration of the target substance.
3. The sample test method of claim 1 ,
wherein the measuring the optical characteristic value of the target substance comprises:
measuring optical characteristic values of the target substance at a main wavelength and a sub-wavelength; and
subtracting the optical characteristic value at the sub-wavelength from the optical characteristic value at the main wavelength.
4. The sample test method of claim 1 , wherein the sample includes blood, and
wherein the target substance includes gamma-glutamyl transferase (GGT).
5. The sample test method of claim 1 , wherein the interfering substance includes bilirubin.
6. The sample test method of claim 1 , wherein the measuring the optical characteristic value of the interfering substance is done without presence of a reagent for reacting with the interfering substance.
7. The sample test method according to claim 1 , wherein the optical characteristic value of the target substance is representative of concentration amount of the target substance and the optical characteristic value of the interfering substance is representative of concentration amount of the interfering substance, and
the sample test method is performed using one or more processors.
8. A test device comprising:
a measurer configured to measure an optical characteristic value of a target substance present in a sample and an optical characteristic value of an interfering substance present in the sample; and
a data processor configured to determine a concentration of the target substance for which interference of the interfering substance is compensated for based on the optical characteristic value of the interfering substance,
wherein the data processor is configured to determine the concentration of the target substance based on the optical characteristic value of the target substance, and determine the concentration of the interfering substance based on the optical characteristic value of the interfering substance, and compensate the concentration of the target substance using the concentration of the interfering substance.
9. The test device of claim 8 , wherein the data processor is configured to apply a fluctuation coefficient to the concentration of the interfering substance to obtain an application result, and then subtract or add the application result from or to the concentration of the target substance.
10. The test device of claim 8 ,
wherein the measurer is configured to the measure optical characteristic value of the target substance at a main wavelength and a sub-wavelength, and
wherein the data processor is configured to subtract the optical characteristic value measured at the sub-wavelength from the optical characteristic value measured at the main wavelength.
11. The test device of claim 8 , wherein the sample includes blood, and
wherein the target substance includes gamma-glutamyl transferase (GGT).
12. The test device of claim 11 , wherein the interfering substance includes bilirubin.
13. The test device of claim 8 ,
wherein the measurer is configured to measure optical characteristic value of the sample at a plurality of wavelengths, and
wherein the data processor is configured to determine a final optical characteristic value as a difference between two or more of the measured optical characteristic values which were measured at a plurality of wavelengths,
wherein the final optical characteristic value is an optical characteristic value for the interfering substance.