Functional ligands to target molecules
The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules, such as amino acids, such as citrulline, and to target molecules such as crystallin or its subunits, such as alpha crystallin B-chain (CRYAB).
1. An artificial ligand binding to citrulline comprising a non-naturally occurring nucleic acid sequence of under 40 nucleotides in length having substantial homology and greater than 80% sequence identity to a sequence selected from the group consisting of SEQ IDs 2-10.
2. The artificial ligand of claim 1 wherein at least one nucleotide is substituted with a non-natural analog.
3. The artificial ligand of claim 1 further comprising an appended 5′-sequence selected from the group consisting of SEQ IDs 231-232.
4. The artificial ligand of claim 1 further comprising an appended 3′-sequence selected from the group consisting of SEQ IDs 231-232.
5. The artificial ligand of claim 1 further comprising an appended 5′-sequence and an appended 3′-sequence selected from the group consisting of SEQ IDs 231-232.
6. An artificial ligand binding to citrulline consisting essentially of a non-naturally occurring nucleic acid sequence having the sequence selected from the group consisting of SEQ IDs 1-10.
7. The artificial ligand of claim 6 wherein at least one nucleotide is substituted with a non-natural analog.
8. The artificial ligand of claim 6 wherein said non-naturally occurring nucleic acid sequence is appended with a 5′-sequence selected from the group consisting of SEQ IDs 231-232.
9. The artificial ligand of claim 6 wherein said non-naturally occurring nucleic acid sequence is appended with a 3′-sequence selected from the group consisting of SEQ IDs 231-232.
10. The artificial ligand of claim 6 wherein said non-naturally occurring nucleic acid sequence is appended with a 5′-sequence and a 3′-sequence selected from the group consisting of SEQ IDs 231-232.