Probiotic dietary supplement formulation
Dietary supplements comprising one or more probiotic bacteria or probiotic yeast in an oil with calcium phosphate added are provided. The calcium phosphate substantially reduces clumping and/or settling of the probiotic in oil. Other additives, such as anti-oxidants and taste enhancers, may be added to the compositions.
1. A dietary supplement comprising an oil-based formulation, wherein:
(a) one or more species or strains of probiotic bacteria; and
(b) dibasic calcium phosphate are dispersed in an oil, in an amount that reduces clumping or settling of the probiotic compared to without the dibasic calcium phosphate.
2. The dietary supplement of claim 1 , wherein the probiotic bacteria is selected from the group consisting of Bacillus subtilis, Bacillus coagulans, Bifidobacterium adolescentis, Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium thermophilum, Lactobacillus acidophilus, Lactobacillus agilis, Lactobacillus alactosus, Lactobacillus alimentarius, Lactobacillus amylophilus, Lactobacillus amylovorans, Lactobacillus amylovorus, Lactobacillus animalis, Lactobacillus batatas, Lactobacillus bavaricus, Lactobacillus bifermentans, Lactobacillus bifidus, Lactobacillus brevis, Lactobacillus buchnerii, Lactobacillus bulgaricus, Lactobacillus catenaforme, Lactobacillus casei, Lactobacillus cellobiosus, Lactobacillus collinoides, Lactobacillus confusus, Lactobacillus coprophilus, Lactobacillus coryniformis, Lactobacillus corynoides, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus delbrueckii, Lactobacillus desidiosus, Lactobacillus divergens, Lactobacillus enterii, Lactobacillus farciminis, Lactobacillus fermentum, Lactobacillus frigidus, Lactobacillus fructivorans, Lactobacillus fructosus, Lactobacillus gasseri, Lactobacillus halotolerans, Lactobacillus helveticus, Lactobacillus heterohiochii, Lactobacillus hilgardii, Lactobacillus hordniae, Lactobacillus inulinus, Lactobacillus jensenii, Lactobacillus jugurti, Lactobacillus kandleri, Lactobacillus kefir, Lactobacillus lactis, Lactobacillus leichmannii, Lactobacillus lindneri, Lactobacillus malefermentans, Lactobacillus mall, Lactobacillus maltaromicus, Lactobacillus minor, Lactobacillus minutus, Lactobacillus mobilis, Lactobacillus murinus, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacillus pseudoplantarum, Lactobacillus reuteri, Lactobacillus rhamnosus, Lactobacillus rogosae, Lactobacillus tolerans, Lactobacillus torquens, Lactobacillus ruminis, Lactobacillus sake, Lactobacillus salivarius, Lactobacillus sanfrancisco, Lactobacillus sharpeae, Lactobacillus trichodes, Lactobacillus vaccinostercus, Lactobacillus viridescens, Lactobacillus vitulinus, Lactobacillus xylosus, Lactobacillus yamanashiensis, Lactobacillus zeae, Pediococcus acidilactici, Pediococcus pentosaceus, Streptococcus cremoris, Streptococcus diacetylactis, Streptococcus ( Enterococcus ) faecium, Streptococcus intermedius, Streptococcus lactis, Streptococcus thermophilus, Saccharomyces boulardii , and combinations thereof.
3. The dietary supplement of claim 2 , wherein the oil is selected from the group consisting of medium-chain triglycerides, soybean oil, safflower oil, corn oil, palm oil, canola oil, or other vegetable oils, mineral oils or combinations thereof.
4. The dietary supplement of claim 1 , wherein the probiotic bacteria is B. infantis, B. breve or combinations thereof and the oil is a medium-chain triglyceride.
5. The dietary supplement of claim 4 , further comprising d-alpha-tocopherol and citric acid.
6. The dietary supplement of claim 1 , wherein the dietary supplement comprises between about 85% to 95% by weight oil, between about 1% to 14.5% by weight of probiotic, and between about 0.5% to 3% by weight dibasic calcium phosphate.
7. The dietary supplement of claim 5 , wherein a 0.5 ml serving consists essentially of between about 20 to 30 mg of probiotic bacteria powder, between about 300 to 500 mg oil, between about 2 to 5 mg dibasic calcium phosphate, between about 0.05 to 0.3 mg of a tocopherol, and between about 0.003 to 0.006 mg citric acid.
8. The dietary supplement of claim 5 , wherein a 0.5 ml serving consists essentially of:
about 25 mg B. infantis M-63 (Moringa strain), providing 1 billion viable cells per serving;
about 488 mg medium chain triglyceride oil;
about 5 mg dibasic calcium phosphate;
about 0.1 mg d-alpha-tocopherol;
and about 0.005 mg citric acid.
9. The dietary supplement of claim 1 , wherein the dibasic calcium phosphate substantially reduces clumping and settling of the probiotic in the oil when compared to probiotic powders in oil without dibasic calcium phosphate added.
10. The dietary supplement of claim 1 , wherein the dibasic calcium phosphate is anhydrous.
11. The dietary supplement of claim 1 , wherein the probiotic bacteria is suspended in a medium-chain triglyceride oil.
12. The dietary supplement of claim 1 , wherein the dietary supplement is non-aqueous.