Porcine animals lacking expression of functional alpha 1, 3 galactosyltransferase
The present invention is a porcine animal, tissue, organ, cells and cell lines, which lack any expression of functional alpha 1,3 galactosyltransferase (alpha1,3GT). These animals, tissues, organs and cells can be used in xenotransplantation and for other medical purposes.
1. A method for producing a porcine animal lacking expression of galactose alpha1,3-galactose, comprising: (a) exposing a population of porcine cells to Clostridium difficile ( C. difficile ) toxin A; (b) removing porcine cells that have an altered morphology upon exposure to C. difficile toxin A; (c) expanding and maintaining a porcine cell that does not have an altered morphology upon exposure to C. difficile toxin A to produce an expanded population of porcine cells; and (d) producing a porcine animal from one or more cells from the expanded population of porcine cells.
2. The method of claim 1 , wherein porcine cells that have an altered morphology appear rounder as compared to the porcine cell that does not have an altered morphology.
3. The method of claim 1 , wherein the population of porcine cells are on a plate matrix prior to exposure to the C. difficile toxin A.
4. The method of claim 3 , wherein the porcine cells that have an altered morphology are released from the plate matrix upon exposure to the C. difficile toxin A.