Compositions comprising bacterial strains
The invention provides compositions comprising one or more bacterial strains for treating preventing diarrhea and/or constipation.
1. A method of treating diarrhea, constipation, or diarrhea and constipation in a subject in need thereof, comprising administering to the subject a pharmaceutical composition comprising:
a bacteria strain of Blautia hydrogenotrophica sufficient to treat the diarrhea, constipation, or diarrhea and constipation in the subject; and
a pharmaceutically acceptable excipient, diluent, or carrier,
wherein the bacteria strain comprises a 16S rRNA gene sequence of SEQ ID NO: 5,
and
wherein the administering results in a reduction in the diarrhea, constipation, or diarrhea and constipation in the subject.
2. The method of claim 1 , wherein the bacteria strain is active.
3. The method of claim 1 , wherein the pharmaceutical composition is encapsulated.
4. The method of claim 1 , wherein the pharmaceutical composition is lyophilized.
5. The method of claim 1 , wherein the pharmaceutical composition is formulated for oral delivery.
6. The method of claim 5 , wherein the pharmaceutical composition is enterically coated.
7. The method of claim 1 , wherein the pharmaceutical composition comprises from about 1×10 3 to about 1×10 11 colony forming units (CFU)/g of the bacteria strain with respect to a total weight of the pharmaceutical composition.
8. The method of claim 1 , wherein the subject has diarrhea and constipation.
9. The method of claim 1 , wherein the pharmaceutical composition comprises an amount sufficient to reduce an amount of hydrogen in the breath of the subject as determined by an administration of lactulose followed by a hydrogen breath analysis.
10. The method of claim 1 , wherein the administering comprises providing one or more doses of 1 g, 3 g, 5 g or 10 g of the pharmaceutical composition.
11. The method of claim 1 , wherein at least 50% of the bacteria strain as measured by an amount of colony forming units (CFU) remains viable after about 1 year of storage when the pharmaceutical composition is stored in a closed container at 25° C. at 95% relative humidity.