Analyte detection
This disclosure relates generally to detection and, in particular, to detecting objects within a sample or fraction thereof.
1. A method of preferentially labeling an extracellular epitope of a biomarker of a cell, comprising:
Permeabilizing the cell;
Preferentially labeling the extracellular epitope of the biomarker of the cell with at least one stain, wherein the at least one stain comprises an affinity molecule bound to a quantum dot with a hydrophilic linker, wherein the cell comprises both the intracellular epitope and the extracellular epitope of the biomarker.
2. The method of claim 1 , wherein the quantum dot has a diameter that is greater than or equal to 2 nanometers.
3. The method of claim 2 , wherein the quantum dot has a hydrophilic shell.
4. The method of claim 3 , wherein the shell is polyeythlene glycol.
5. The method of claim 1 , further comprising performing antigen retrieval on the cell, wherein the antigen retrieval step is performed after permeabilizing and before the adding step.
6. The method of claim 5 , wherein the antigen retrieval step is performed at 70-100° C.
7. The method of claim 6 , wherein the antigen retrieval step is performed at 70-90° C.