Eicosapentaenoic acid-producing microorganisms, fatty acid compositions, and methods of making and uses thereof

1. A microbial oil comprising omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid and eicosapentaenoic acid in an amount of >90%, by weight, of the total amount of omega-3 polyunsaturated fatty acids, wherein the amount of eicosapentaenoic acid, by weight, is from 19% to 55% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and the amount of docosahexaenoic acid, by weight, is from 35% to 71% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and wherein the oil comprises from 120 mg to 220 mg eicosapentaenoic acid per one gram of oil and from 240 mg to 450 mg docosahexaenoic acid per one gram of oil, and wherein said microbial oil further comprising an effective amount of at least one added antioxidant.

2. A microbial oil comprising omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid and eicosapentaenoic acid in an amount of ≥90%, by weight, of the total amount of omega-3 polyunsaturated fatty acids, wherein the amount of eicosapentaenoic acid, by weight, is from 19% to 55% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and the amount of docosahexaenoic acid, by weight, is from 35% to 71% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and wherein the oil comprises from 130 mg to 195 mg eicosapentaenoic acid per one gram of oil and from 320 mg to 480 mg docosahexaenoic acid per one gram of oil, and wherein said microbial oil further comprising an effective amount of at least one added antioxidant.

3. A microbial oil comprising omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid and eicosapentaenoic acid in an amount of ≥90%, by weight, of the total amount of omega-3 polyunsaturated fatty acids, wherein the amount of eicosapentaenoic acid, by weight, is from 10% to 25% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and the amount of docosahexaenoic acid, by weight, is from 75% to 90% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and where the omega-3 polyunsaturated fatty acids further comprise docosapentaenoic acid in an amount of from 0% to 2%, by weight, of the total amount of omega-3 polyunsaturated fatty acids, and wherein said microbial oil further comprising an effective amount of at least one added antioxidant.

4. The oil of claim 3 , wherein the total amount of omega-3 polyunsaturated fatty acids in the microbial oil is at least about 400 mg per gram of oil.

5. A microbial oil comprising omega-3 polyunsaturated fatty acids comprising docosahexaenoic acid and eicosapentaenoic acid in an amount of about >90%, by weight, of the total amount of omega-3 polyunsaturated fatty acids, wherein the amount of eicosapentaenoic acid, by weight, is from about 19% to about 55% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, and the amount of docosahexaenoic acid, by weight, is from about 35% to about 71% of the total amount of docosahexaenoic acid and eicosapentaenoic acid, wherein the total amount of omega-3 polyunsaturated fatty acids is at least about 400 mg per one gram of oil, and wherein said microbial oil further comprising an effective amount of at least one added antioxidant.

6. The oil of any one of claims 1 - 5 , wherein said oil is produced by Schizochytrium sp.

7. The oil of any one of claims 1 - 5 , wherein said oil comprises less that about 5% by weight of each of arachidonic acid, docosapentaenoic acid n-6, oleic acid, linoleic acid, linolenic acid, eicosenoic acid, erucic acid and stearidonic acid.

8. A microbial oil comprising a triacylglycerol fraction of at least 10% by weight, wherein at least about 12% by weight of the fatty acids in the triacylglycerol fraction is eicosapentaenoic acid, wherein at least 25% by weight of the fatty acids in the triacylglycerol fraction is docosahexaenoic acid, wherein said oil has a peroxide value of 5 or less, and wherein said microbial oil further comprising an effective amount of at least one added antioxidant.

9. The oil of any one of claims 1 - 5 , wherein said oil comprises a ratio of EPA:DHA selected from 1:1 to 1:1.5, 1:1 to 1:2, 1:1.5 to 1:2, 1:1 to 1:2.5, 1:2 to 1:2.5, and 1:4 to 1:7 by weight of total omega-3 polyunsaturated fatty acids.

10. A microbial oil comprising 120 mg to 220 mg eicosapentaenoic acid per one gram of oil and from 240 mg to 450 mg docosahexaenoic acid per one gram of oil, and wherein said microbial oil further comprising an effective amount of at least one added antioxidant.

11. The oil of claim 9 , wherein said oil is produced by Schizochytrium sp.