ENHANCED PLANTS OF GENUS HUMULUS AND METHODS OF MAKING AND USING THE SAME
Disclosed herein is a genetically modified plant of genus humulus not requiring flowering for producing lupulin glands comprising secondary compounds. Provided herein is a new plant of genus humulus having lupulin glands on non-flowering parts of the plant, such as leaves. The disclosed plants have a high mass % of secondary compounds and a high degree of lupulin glands coverage on the surface of the plant. Also disclosed herein are methods of producing secondary compounds from a plant of genus humulus without flowering the plant of genus humulus. For example, the disclosed methods provide for inducing lupulin glands development on a plant of genus humulus without flowering the plant of genus humulus.
1 . A plant of genus humulus, comprising;
lupulin glands on non-flowering parts of the plant of genus humulus;
a vector comprising genetic information; and
wherein the plant of genus humulus comprises a surface area.
2 . The plant of claim 1 , wherein the vector is a plasmid comprising a cDNA sequence native to a plant of genus humulus corresponding to lupulin gland induction.
3 . The plant of claim 1 , wherein the vector is a purified transformed bacteria comprising a DNA sequence native to a plant of genus humulus, wherein said native DNA sequence corresponds to lupulin gland induction.
4 . The plant of claim 1 , comprising lupulin glands on about 25-100% of the surface area of the plant.
5 . The plant of claim 4 , comprising lupulin glands on about 50-100% of the surface area of the plant.
6 . The plant of claim 4 , comprising lupulin glands on about 70-100% of the surface area of the plant.
7 . The purified transformed bacteria of claim 1 , comprising a buffer solution; wherein the vector is suspended in the buffer solution.
8 . The plant of claim 1 , comprising 20-80 mass % of secondary compounds of the plant.
9 . The plant of claim 8 , comprising about 30-70 mass % of secondary compounds of the plant.
10 . The plant of claim 8 , comprising about 40-65 mass % of secondary compounds of the plant.
11 . The plant of claim 8 , comprising about 50-60 mass % of secondary compounds of the plant.
12 . A plant of the genus humulus comprising cDNA fragments corresponding to lupulin gland induction in a plant not of the genus humulus.
13 . The plant of claim 12 , wherein the plant not of the genus humulus is a plant of genus Arabidopsis.
14 . A method of producing secondary compounds, comprising inducing lupulin gland development in a plant of genus humulus.
15 . The method of claim 14 , wherein said secondary compounds are chosen from alpha acids, beta acids, essential oils, flavonoids, or terpenes.
16 . The method of claim 15 , comprising harvesting at least one alpha acid or at least one terpene during the vegetative growth cycle of the plant of the genus humulus.
17 . The method of claim 14 , comprising modifying genetic material of the plant of the genus humulus.
18 . The method of claim 14 , comprising introducing non-native DNA sequence into the plant of the genus humulus.
19 . The method of claim 17 , comprising overexpressing at least one lupulin gland gene.
20 . The method of claim 19 , comprising interfering with expression of at least one lupulin gland gene.
21 . The method of claim 20 , wherein the at least one lupulin gland gene is chosen from a bHLH Basic Helix Loop Helix transcription factor, a R2R3 MYB transcription factor, a R3 MYB transcription factor, a WD40 repeat transcription factor/ protein, or a homeodomain transcription factor.