Refillable column system
The invention is directed to a column system for separation of particles comprising a reservoir section with a input opening; a filter section provided with a filter matrix and an output opening and a plunger having a first and a second end, the first end fitting into the reservoir section characterized in that the plunger is provided with at least one channel providing gaseous communication from the first end to the second end. The column system may be used for magnetic cell separation.
1. A column system for separation of particles comprising:
a reservoir section with an input opening;
a filter section provided with a filter matrix and an output opening, wherein the filter matrix is a ferromagnetic matrix; and
a plunger having a first and a second end, the first end fitting into the reservoir section wherein the plunger is provided with at least one channel providing gaseous communication from the first end to the second end.
2. The column system according to claim 1 , wherein the channel is provided at the second end with a closing means.
3. The column system according to claim 1 , wherein the plunger is provided with a means to adjust the distance to which the first end of the plunger is inserted into the reservoir section.
4. The column system according to claim 1 , wherein the plunger is provided with stabilizer means fitting into the reservoir section.
5. The column system according to claim 1 , wherein the plunger is provided with a sealing means at first end.
6. The column system according to claim 1 , wherein the plunger is provided with a flip-cap, a screw cap or an adhesive film as closing means.
7. A process for separation of particles using a column comprising a reservoir section with a input opening; a filter section provided with a filter matrix and an output opening and a plunger having a first and a second end, the first end fitting into the reservoir section wherein the plunger is provided with at least one channel providing gaseous communication from the first end to the second end, comprising:
providing a suspension of particles to the reservoir section;
inserting the first end of the plunger into the input opening of the column section with the channel being closed;
moving the plunger towards the filter matrix thereby pressing the suspension through the filter matrix; and
opening the channel and removing the plunger from the column.
8. The process according to claim 7 , wherein the channel is opened or closed with a closing means provided at the second end of the plunger.
9. The process according to claim 7 , wherein the filter matrix is a ferromagnetic matrix and the process is performed at least in part by placing the filter section of the column in a magnetic field.