Modified glucosyltransferases for producing branched alpha-glucan polymers
Glucosyltransferase enzymes are disclosed herein that produce branched alpha-glucan polymer. Also disclosed, for example, are polynucleotides encoding these enzymes, as well as methods of producing branched alpha-glucan polymer.
1. A glucosyltransferase enzyme comprising an amino acid sequence that is at least 97% identical to: SEQ ID NO:85, SEQ ID NO:89, or SEQ ID NO:93, wherein said glucosyltransferase enzyme lacks the amino acid sequence of SEQ ID NO:78 or SEQ ID NO:80, and wherein said glucosyltransferase enzyme produces a branched alpha-glucan polymer.
2. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:85, SEQ ID NO:89, or SEQ ID NO:93.
3. A reaction solution comprising water, sucrose, and a glucosyltransferase enzyme according to claim 1 .
4. A method for producing a branched alpha-glucan polymer comprising:
(a) contacting at least water, sucrose, and a glucosyltransferase enzyme according to claim 1 , whereby branched alpha-glucan polymer is produced, and
(b) optionally, isolating the branched alpha-glucan polymer produced in step (a).
5. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:85.
6. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:85.
7. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:89.
8. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:89.
9. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 97% identical to SEQ ID NO:93.
10. The glucosyltransferase enzyme of claim 1 , wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 98% identical to SEQ ID NO:93.