DNA vaccines encoding heat shock proteins
View Patent ↗A method of treating a T cell-mediated inflammatory autoimmune disease by administering to an individual in need thereof an immunogenic composition comprising a recombinant construct of a nucleic acid sequence encoding heat shock protein 90 (HSP 90), or an active fragment thereof, wherein the nucleic acid sequence is operatively linked to one or more transcription control sequences. The disease is other than insulin dependent diabetes mellitus (IDDM) or rheumatoid arthritis. The administering of the immunogenic composition results in a shift of the immune response to a Th2 response, thereby treating the disease.
1. A method of treating a T cell-mediated inflammatory autoimmune disease in an individual, the method comprising:
administering by intramuscular injection to an individual having a T cell-mediated inflammatory autoimmune disease a composition comprising a nucleic acid molecule encoding a mammalian heat shock protein 90 (HSP90), or an active fragment thereof, in an amount to provide from about 0.1 μg/kg body weight to about 200 μg/kg body weight of the nucleic acid molecule encoding the mammalian HSP90, or an active fragment thereof to the individual, wherein the nucleic acid molecule is operatively linked to one or more transcription control sequences,
wherein the administering treats the T cell-mediated inflammatory autoimmune disease in the individual.
2. The method of claim 1 , wherein the composition comprises a delivery vehicle selected from the group consisting of liposomes, micelles, and emulsions.
3. The method of claim 1 , wherein the nucleic acid molecule encodes mammalian HSP 90.
4. The method of claim 3 , wherein the mammalian HSP90 is human HSP90.
5. The method of claim 4 , wherein the individual is a human.
6. The method of claim 5 , wherein the T cell-mediated inflammatory autoimmune disease is inflammatory bowel disease and wherein the composition is administered in an amount to provide about 0.1 μg/kg body weight to about 10 μg/kg body weight of the nucleic acid molecule encoding the human HSP90.
7. The method of claim 5 , wherein the T cell-mediated inflammatory autoimmune disease is ulcerative colitis.
8. The method of claim 5 , wherein the T cell-mediated inflammatory autoimmune disease is Crohn's disease.
9. The method of claim 1 , wherein the composition is administered in an amount to provide about 0.5 μg/kg body weight to about 200 μg/kg body weight of the nucleic acid molecule encoding the mammalian HSP90, or an active fragment thereof to the individual.
10. The method of claim 9 , wherein the nucleic acid molecule encodes mammalian HSP 90.
11. The method of claim 10 , wherein the mammalian HSP90 is human HSP90.
12. The method of claim 11 , wherein the individual is a human.
13. The method of claim 6 , wherein the inflammatory bowel disease is ulcerative colitis.
14. The method of claim 6 , wherein the inflammatory bowel disease is Crohn's disease.
15. The method of claim 1 , wherein the composition is administered in an amount to provide about 0.1 μg/kg body weight to about 10 μg/kg body weight of the nucleic acid molecule encoding the mammalian HSP90, or an active fragment thereof to the individual.